Fig. 8
To confirm RNA-Seq determined fold changes, NanoString nCounter Analysis was performed. For complete target and probe information used for confirmation see Additional file 3: Table S3. Panel (a) Log2(Fold Change) values for each 50 nm waveband exposure were determined by each independent technology and plotted. Analysis of the 350–400 nm region (50 transcripts) is in orange, the 400–450 nm region (22 transcripts) is in gray, the 450–500 nm region (19 transcripts) is in yellow, the 500–550 nm region (59 transcripts) is in light blue and the 550–600 nm region (26 transcripts) is in green. R2 values were calculated for each correlation and determined to be 0.88, 0.84, 0.90, 0.98 and 0.81 respectively for each of the 50 nm region. In addition, analysis of the correlation indicated that 97% of the transcripts tested across all wavebands were confirmed in direction. Panel (b) To confirm RNA-Seq determined fold changes, NanoString nCounter Analysis was performed. The plot shows log2(Fold Change) values determined for each 10 nm waveband exposure by each independent technology. Analysis of the 500–510 nm region (30 transcripts) is in dark blue, the 510–520 nm region (37 transcripts) is in orange, the 520–530 nm region (24 transcripts) is in gray, the 530–540 nm region (32 transcripts) is in yellow and the 540–550 nm region (23 transcripts) is in light blue. R2 values were calculated for each correlation and determined to be 0.71, 0.81, 0.86, 0.87 and 0.85, respectively for the 10 nm region. Analysis of the correlations indicate 94% of the transcripts tested across all wavebands confirmed in the direction of modulated expression