Fig. 1
Volcano plots of the transcriptional responses of clone G to temperature changes. Contrasts of the transcriptional responses of the D. pulex clone G to (a-c) 2, 4, and 8 h under acute heat stress and control conditions (30 °C vs. 20 °C) or (d-f) temperature acclimation (20 °C vs. 10 °C, 24 °C vs. 20 °C, 24 °C vs. 10 °C) were depicted as statistical significance levels (−log10 Q values) versus expression changes (log2-fold changes). The green or red numbers on top are numbers of down- or upregulated differentially expressed genes (DEGs), which are defined by a Q (FDR) value ≤0.05 (the horizontal dashed lines show the position of -log10 (0.05) on the Y axis). The numbers directly below are numbers of KOG-identified DEGs (KOGs). The green and red numbers even lower down, which are on the left or right of the vertical dashed lines, are numbers of DEGs and KOG-identified DEGs with log2-fold changes < − 2 or > 2. For all experimental conditions, four biological replicates (50 animals each) were used