Fig. 10
Two-dimensional protein gels from clone G under acute heat stress. The 2D gels, which are fusion (averaged) images from a varying number (n) of gels (biological replicates, 25-30 animals each), show changes in protein expression in the D. pulex clone G after the acute exposure of control animals (20 °C, ad libitum feeding) (blue spots; n = 5) to (a) 24 h (orange spots; n = 4) or (c) 48 h (orange spots; n = 6) of heat stress (30 °C, ad libitum feeding). Red or green spot IDs mark significantly up- or downregulated proteins (t-tests, P < 0.05; see Table 3). The scatter plots show changes in expression levels (Vrelative, relative spot volume) between control and heat-stressed animals (b, 24 h; d, 48 h) of significantly (large circles and letters) or non-significantly (small circles) up- or downregulated proteins (data from a or c). Proteins, which were upregulated under heat stress, are shown above the diagonal line. Spot IDs and functions are from Table 3. For comparison reasons (see Fig. 14), the expression levels of the non-significantly regulated protein ID383 (JGI gene ID: 301074, Chaperonin ATPase, Cpn60/Hsp60p; e.g., Table 3) are also shown (yellow circles). Abbreviations for Figs. 10, 11, 12, 15, and Additional files 3, 4, 5, 6: Figures S2-S5: 3-phosphoglycerate kinase (3-P), 20S proteasome (20S), actin (Act), α-tubulin (α-T), arginine kinase (Arg), β-glucosidase (β-G), β-tubulin (β-T), calreticulin (Cal), carboxypeptidase (Car), chaperonin ATPase (HSP60), cytosolic fatty-acid binding protein (Cyt), enolase (Eno), FKBP-type peptidyl-prolyl cis-trans isomerase (FKBP), FOG, leucine-rich repeat (FOG), glutathione transferase (Glu), glyceraldehyde 3-phosphate dehydrogenase (G3pd), glycoside hydrolase (Gly), H+-transporting two-sector ATPase (H+), M13 family peptidase (M13), peptidase S1 (Pep), protein disulfide isomerase (Pdi), serine endopeptidase (Ser), ubiquitin (Ubi), vitellogenin (Vit)