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Fig. 14 | BMC Genomics

Fig. 14

From: The transcriptomic and proteomic responses of Daphnia pulex to changes in temperature and food supply comprise environment-specific and clone-specific elements

Fig. 14

Expression of the heat shock protein HSP60 in the D. pulex clones G and M under heat stress. Expression of the heat shock protein HSP60 was determined in the D. pulex clones G and M under heat stress by western blot analyses using control animals (20 °C, ad libitum feeding) and animals exposed to 24 h or 48 h of heat stress (30 °C, ad libitum feeding). a Representative gel showing for clone G (left) and clone M (right) intensity changes of two HSP60 bands (at 73.8 and 60.4 kDa) under control (ctrl) and heat stress conditions (24 h, 48 h), with the protein marker at the center and heat-shocked HeLa cells as positive controls. b Mean intensities (Daphnia HSP60 vs. HeLa HSP60) ± standard deviation (n = 3 experiments per clone and condition) of the 60.4 kDa or 73.8 kDa (dotted filling pattern) bands from clone G (white bars) or clone M (gray bars) under control or stress conditions. c Ratios (i.e., expression changes of HSP60 in the contrast 30 °C vs. 20 °C) from western blot analysis (60.4 kDa HSP 60) or from proteomics (diagonal filling pattern; see Figs. 10, 11) for clone G (white bars) or M (gray bars). Small letters indicate significant differences between animals of clone M after 24 h of heat stress and control animals of both clones (a, b; P < 0.05, one-way anova and Student-Newman-Keuls multiple comparison procedure)

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