The transcriptomic and proteomic responses of Daphnia pulex to changes in temperature and food supply comprise environment-specific and clone-specific elements

Table 2 Protein concentrations in the D. pulex clones G and M

Clone	Stress	Time (h)	Protein concentration
G	Control	0	24.3 ± 5.2
G	30 °C, ad libitum feeding	24	25.0 ± 5.3
G	Starvation, 20 °C	24	14.9 ± 2.1^*
G	30 °C, starvation	24	16.6 ± 4.7
G	30 °C, ad libitum feeding	48	24.0 ± 4.2
G	Starvation, 20 °C	48	12.0 ± 1.8^**
G	30 °C, starvation	48	–
M	Control	0	25.6 ± 3.8
M	30 °C, ad libitum feeding	24	25.0 ± 5.0
M	Starvation, 20 °C	24	18.0 ± 16.7
M	30 °C, starvation	24	23.0 ± 5.1
M	30 °C, ad libitum feeding	48	22.1 ± 4.7
M	Starvation, 20 °C	48	21.0 ± 3.1
M	30 °C, starvation	48	22.2 ± 6.6

Protein concentration (μg/mg fresh weight; mean ± standard deviation; per clone, stress condition, and period, n = 3-10 measurements on N = 25-30 animals each) in control animals or differently stressed animals (heat, starvation, heat-and-starvation) of the D. pulex clones G and M after different periods of stress exposure (24 h or 48 h). Asterisks indicate significant differences compared with control conditions (20 °C, ad libitum feeding): *, P < 0.05; **, P < 0.01 (t-tests)

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