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Table 3 Differentially expressed proteins (DEPs) in the D. pulex clones G and M under heat stress

From: The transcriptomic and proteomic responses of Daphnia pulex to changes in temperature and food supply comprise environment-specific and clone-specific elements

Spot ID Gene ID Function R P R P SP Mre Mrp pIe pIp SC (%) NP
  24 h 48 h  
743 306433 20S Proteasome, A and B subunits 0.5 0.00 0.7 0.14 34.3 28 5.3 5.3 35.9 8
520 300012 Actin and related proteins 3.1 0.06 2.4 0.04 62.6 41.8 5.5 5.3 30.0 8
3309 306442 Actin and related proteins 1.3 0.03 1.4 0.00 62.6 41.9 5.4 5.2 30.8 8
417 100611 α-Tubulin 2.2 0.02 2.0 0.00 82.3 46.1 5.4 5.0 23.8 8
405 301837 α-Tubulin 2.1 0.00 1.7 0.01 81.9 49.9 5.3 5.0 33.0 16
416 301837 α-Tubulin 1.9 0.00 1.7 0.02 81.9 49.9 5.2 5.0 11.8 6
757 220693 Arginine kinase 0.9 0.35 1.5 0.01 28.8 39.9 5.4 5.5 36.3 9
796 220693 Arginine kinase 0.6 0.03 0.6 0.01 24.4 39.9 5.1 5.5 34.9 10
438 300845 β-Tubulin 1.6 0.01 1.3 0.09 78.8 50.1 5.1 4.7 19.9 10
463 210624 Calreticulin 1.7 0.03 1.2 0.44 16 72.4 46.6 4.7 4.5 48.3 18
742 303899 Carboxypeptidase A2 0.8 0.06 0.6 0.01 17 30.0 46.1 4.8 5.2 29.6 14
609 304126 FOG, leucine-rich repeat 1.7 0.00 2.0 0.00 22 47.2 42.1 4.6 4.7 18.6 7
801 303282 Glutathione transferase 1.3 0.07 1.6 0.00 24.2 24.8 5.3 5.4 36.8 12
794 317266 Glutathione transferase 1.0 0.84 1.4 0.05 25.0 25 5.4 5.2 67.3 18
472 300366 Glycoside hydrolase, family 7 0.5 0.07 0.5 0.02 19 70.2 50.3 4.8 4.8 21.5 8
600 303,036 Glycoside hydrolase, family 16 0.3 0.01 0.3 0.00 19 47.5 40.4 4.9 4.8 30.1 12
441 309746 H+-transporting two-sector ATPase 1.9 0.00 1.4 0.07 78.1 56.8 5.2 5.5 43.8 16
466 309746 H+-transporting two-sector ATPase 2.2 0.00 1.8 0.00 71.4 56.8 5.2 5.5 79.4 56
23,222 309746 H+-transporting two-sector ATPase 0.5 0.09 0.5 0.02 66.8 56.8 5.1 5.5 65.5 23
293 9558 Ubiquitin and ubiquitin-like proteins 0.8 0.63 0.4 0.04 107.3 5.7 5.1 5.2 35.3 2
845 9558 Ubiquitin and ubiquitin-like proteins 0.9 0.59 0.6 0.01 18.3 5.7 4.9 5.2 35.3 2
937 308693 Vitellogenin 1.4 0.08 1.4 0.05 20 12.1 191.9 4.7 6.5 11.3 16
Spot ID Gene ID Function R P R P SP Mre Mrp pIe pIp SC (%) NP
  24 h 48 h  
520 300012 Actin and related proteins 0.5 0.04 0.4 0.00 62.6 41.8 5.5 5.3 30.0 8
724 305550 Actin and related proteins 1.7 0.02 1.5 0.04 32.0 41.8 5.7 5.4 37.9 12
405 301837 α-Tubulin 1.8 0.01 1.5 0.01 81.9 49.9 5.3 5.0 33.0 16
438 300,845 β-Tubulin 1.5 0.01 1.0 0.80 78.8 50.1 5.1 4.7 19.9 10
742 303899 Carboxypeptidase A2 0.5 0.03 0.7 0.05 17 30.0 46.1 4.8 5.2 29.6 14
383 301074 Chaperonin ATPase, Cpn60/Hsp60p 5.1 0.01 3.1 0.04 89.1 61.4 5.6 5.8 31.2 22
908 300446 Cytosolic fatty-acid binding protein 0.5 0.04 0.8 0.25 26.1 14.8 6.0 5.3 31.1 4
609 304126 FOG, leucine-rich repeat 3.0 0.00 5.6 0.00 22 47.2 42.1 4.6 4.7 18.6 7
784 305501 Glutathione transferase 1.6 0.06 1.8 0.03 25.3 25.2 5.8 5.6 36.2 10
794 317266 Glutathione transferase 1.5 0.03 1.3 0.02 25.0 25 5.4 5.2 67.3 18
600 303036 Glycoside hydrolase, family 16 0.5 0.14 0.6 0.03 19 47.5 40.4 4.9 4.8 30.1 12
415 306451 H+-transporting two-sector ATPase 3.2 0.03 2.7 0.02 66.9 55.5 5.7 5.5 57.6 56
779 309746 H+-transporting two-sector ATPase 1.8 0.01 1.6 0.10 26.5 56.8 5.9 5.5 64.2 20
439 234212 Protein disulfide isomerase 2.0 0.00 1.3 0.23 15 78.1 58.3 5.0 4.8 44.4 22
635 251885 Serine endopeptidase 0.4 0.16 0.4 0.01 19 41.5 39.6 4.4 5.0 12.7 6
641 251885 Serine endopeptidase 0.6 0.21 0.4 0.01 19 41.2 39.6 4.5 5.0 12.7 6
270 219769 Vitellogenin fused with SOD 3.4 0.05 4.2 0.02 17 110.9 223 5.6 6.5 12.3 22
301 219769 Vitellogenin fused with SOD 4.4 0.02 5.3 0.00 17 107.0 223 5.6 6.5 13.3 34
305 219769 Vitellogenin fused with SOD 2.5 0.04 3.0 0.00 17 104.8 223 5.5 6.5 12.5 23
322 219769 Vitellogenin fused with SOD 5.1 0.06 9.3 0.01 17 102.6 223 5.6 6.5 12.3 21
576 219769 Vitellogenin fused with SOD 1.6 0.31 3.2 0.05 17 51.9 223 5.7 6.5 7.5 18
580 219769 Vitellogenin fused with SOD 2.2 0.13 2.4 0.04 17 51.8 223 5.6 6.5 9.9 22
689 219769 Vitellogenin fused with SOD 0.9 0.87 0.3 0.01 17 38.1 223 5.7 6.5 12.9 25
1545 219769 Vitellogenin fused with SOD 2.7 0.05 3.3 0.01 17 51.8 223 5.6 6.5 9.9 22
  1. D. pulex clone G on top, D. pulex clone M at the bottom. From left to right: Spot identity (ID), gene ID (JGI_V11_gene ID), protein function (described in the protein sequence database of the Daphnia pulex genome assembly v1.0), expression ratios (R) contrasting stress (24 h or 48 h at 30 °C, ad libitum feeding) and control (20 °C, ad libitum feeding) conditions and associated P values (t-tests), predicted length of N-terminal signal peptides (SP), experimental and predicted molecular masses (Mre, Mrp) and isoelectric points (pIe, pIp) of the mature protein (without signal peptide), sequence coverage (SC; percentage of the predicted protein sequence covered by matching peptide sequences), and number of matching peptides (NP). Bold R and P values indicate statistical significance. Number of gels (biological replicates, 25-30 animals each) for clone G or M: control (5 or 4) and 24 h (4 or 3) or 48 h (6 or 10) of stress