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Table 5 Differentially expressed proteins (DEPs) in the D. pulex clones G and M under heat-and-starvation stress

From: The transcriptomic and proteomic responses of Daphnia pulex to changes in temperature and food supply comprise environment-specific and clone-specific elements

Spot ID

Gene ID

Function

R

P

SP

Mre

Mrp

pIe

pIp

SC (%)

NP

 

24 h

 

501

299795

3-Phosphoglycerate kinase

1.9

0.00

–

83.4

44.2

5.6

5.5

39.4

28

520

300012

Actin and related proteins

1.8

0.05

–

62.6

41.8

5.5

5.3

30.0

8

3309

306442

Actin and related proteins

1.4

0.02

–

62.6

41.9

5.4

5.2

30.8

8

543

220693

Arginine kinase

2.0

0.03

–

58.0

39.9

5.6

5.5

58.4

32

563

220693

Arginine kinase

3.1

0.03

–

57.6

39.9

5.6

5.5

58.4

32

796

220693

Arginine kinase

0.5

0.03

–

24.4

39.9

5.1

5.5

34.9

10

742

303899

Carboxypeptidase A2

0.6

0.02

17

30.0

46.1

4.8

5.2

29.6

14

383

301074

Chaperonin ATPase, Cpn60/Hsp60p

1.8

0.03

–

89.1

61.4

5.6

5.8

31.2

22

801

303282

Glutathione transferase

1.6

0.00

–

24.2

24.8

5.3

5.4

36.8

12

784

305501

Glutathione transferase

1.6

0.03

–

25.3

25.2

5.8

5.6

36.2

10

600

303036

Glycoside hydrolase, family 16

0.3

0.01

19

47.5

40.4

4.9

4.8

30.1

12

779

309746

H+-transporting two-sector ATPase

2.0

0.00

–

26.5

56.8

5.9

5.5

64.2

20

23,222

309746

H+-transporting two-sector ATPase

0.4

0.03

–

66.8

56.8

5.1

5.5

65.5

23

722

248155

Peptidase S1, chymotrypsin

2.0

0.02

15

39.9

30.2

5.5

4.7

22.3

3

845

9558

Ubiquitin and ubiquitin-like proteins

0.6

0.01

–

18.3

5.7

4.9

5.2

35.3

2

448

308693

Vitellogenin

0.5

0.01

20

74.3

191.9

5.5

6.5

9.8

31

Spot ID

Gene ID

Function

R

P

R

P

SP

Mre

Mrp

pIe

pIp

SC (%)

NP

 

24 h

48 h

 

520

300012

Actin and related proteins

0.4

0.00

0.4

0.00

–

62.6

41.8

5.5

5.3

29.97

8

752

300012

Actin and related proteins

1.0

0.73

1.7

0.01

–

28.2

41.8

5.6

5.3

36.34

17

724

305550

Actin and related proteins

2.3

0.01

2.5

0.00

–

32.0

41.8

5.7

5.3

37.93

12

3309

306442

Actin and related proteins

1.4

0.19

1.5

0.03

–

62.6

41.9

5.4

5.2

30.77

8

405

301837

α-Tubulin

1.6

0.02

1.6

0.04

–

81.9

49.9

5.3

5.0

33.04

16

543

220693

Arginine kinase

1.7

0.06

1.9

0.03

–

58.0

39.9

5.6

5.5

58.38

32

563

220693

Arginine kinase

1.4

0.11

2.0

0.00

–

57.6

39.9

5.6

5.5

58.38

32

411

314456

β-Glucosidase

0.9

0.69

0.6

0.03

19

81.9

57.1

5.0

4.9

15.54

9

463

210624

Calreticulin

0.9

0.49

0.5

0.00

16

72.4

46.6

4.7

4.5

48.3

18

742

303899

Carboxypeptidase A2

0.9

0.51

0.5

0.00

17

30.0

46.1

4.8

5.2

29.59

14

383

301074

Chaperonin ATPase, Cpn60/Hsp60p

5.5

0.00

7.1

0.00

–

89.1

61.4

5.6

5.8

31.2

22

908

300446

Cytosolic fatty-acid binding protein

0.5

0.05

0.2

0.00

–

26.1

14.8

6.0

5.3

31.06

4

809

301844

Enolase

1.6

0.02

1.5

0.26

–

26.1

46.8

6.0

5.7

28.11

8

1027

301844

Enolase

1.1

0.53

1.4

0.05

–

5.0

46.8

6.6

5.7

19.82

6

805

231271

FKBP-type peptidyl-prolyl cis-trans isomerase

0.6

0.03

0.8

0.35

17

23.3

23.4

4.5

4.5

66.67

14

609

304126

FOG, leucine-rich repeat

3.2

0.02

3.5

0.00

22

47.2

42.1

4.6

4.7

18.64

7

801

303282

Glutathione transferase

1.7

0.04

1.3

0.13

–

24.2

24.8

5.3

5.4

36.77

12

784

305501

Glutathione transferase

3.0

0.00

3.4

0.00

–

25.3

25.2

5.8

5.6

36.24

10

794

317266

Glutathione transferase

1.6

0.02

1.6

0.04

–

25.0

25

5.4

5.2

67.26

18

600

303036

Glycoside hydrolase, family 16

0.8

0.35

0.5

0.01

19

47.5

40.4

4.9

4.8

30.08

12

415

306451

H+-transporting two-sector ATPase

3.2

0.01

9.1

0.00

–

66.9

55.5

5.7

5.5

57.6

56

466

309746

H+-transporting two-sector ATPase

1.2

0.39

1.7

0.02

–

71.4

56.8

5.2

5.5

79.4

56

779

309746

H+-transporting two-sector ATPase

2.9

0.02

3.1

0.01

–

26.5

56.8

5.9

5.5

64.23

20

23,222

309746

H+-transporting two-sector ATPase

1.0

0.80

0.4

0.00

–

66.8

56.8

5.1

5.5

65.54

23

332

200882

M13 family peptidase

0.6

0.07

0.3

0.00

–

99.0

75.4

4.7

4.6

47.86

34

635

251885

Serine endopeptidase

0.3

0.02

0.3

0.01

19

41.5

39.6

4.4

5.0

12.71

6

641

251885

Serine endopeptidase

0.2

0.01

0.3

0.00

19

41.2

39.6

4.5

5.0

12.71

6

444

9558

Ubiquitin and ubiquitin-like proteins

2.4

0.00

4.8

0.00

–

76.2

5.7

4.6

5.2

35.29

2

845

9558

Ubiquitin and ubiquitin-like proteins

0.7

0.09

0.5

0.00

–

18.3

5.7

4.9

5.2

35.29

2

231

308693

Vitellogenin

3.5

0.08

5.2

0.01

20

117.0

191.9

5.7

6.5

16.34

47

238

308693

Vitellogenin

3.7

0.13

2.9

0.04

20

117.0

191.9

5.8

6.5

18.39

46

270

219769

Vitellogenin fused with SOD

2.5

0.02

4.6

0.02

17

110.9

223

5.6

6.5

12.3

22

301

219769

Vitellogenin fused with SOD

3.9

0.01

3.9

0.00

17

107.0

223

5.6

6.5

13.25

34

305

219769

Vitellogenin fused with SOD

3.8

0.00

2.5

0.01

17

104.8

223

5.5

6.5

12.45

23

322

219769

Vitellogenin fused with SOD

3.0

0.01

3.5

0.00

17

102.6

223

5.6

6.5

12.25

21

325

219769

Vitellogenin fused with SOD

3.8

0.00

4.0

0.00

17

101.9

223

5.6

6.5

12.55

29

576

219769

Vitellogenin fused with SOD

2.4

0.01

3.6

0.00

17

51.9

223

5.7

6.5

7.5

18

580

219769

Vitellogenin fused with SOD

3.3

0.00

7.0

0.00

17

51.8

223

5.6

6.5

9.85

22

616

219769

Vitellogenin fused with SOD

1.3

0.32

2.1

0.01

17

45.1

223

5.6

6.5

8.9

24

689

219769

Vitellogenin fused with SOD

0.7

0.55

0.2

0.02

17

38.1

223

5.7

6.5

12.85

25

1545

219769

Vitellogenin fused with SOD

3.1

0.02

6.7

0.02

17

51.8

223

5.6

6.5

9.85

22

  1. D. pulex clone G on top, D. pulex clone M at the bottom. From left to right: Spot ID, gene ID, protein function, expression ratios (R) contrasting stress (24 h or 48 h at 30 °C, starvation) and control (20 °C, ad libitum feeding) conditions and associated P values (t-tests), predicted length of N-terminal signal peptides (SP), experimental and predicted molecular masses (Mre, Mrp) and isoelectric points (pIe, pIp) of the mature protein, sequence coverage (SC), and number of matching peptides (NP). Bold R and P values indicate statistical significance. (Clone G did not survive 48 h of heat-and-starvation stress.) See Table 3 for details. Number of gels (biological replicates, 25-30 animals each) for clone G or M: control (5 or 4) and 24 h (5 or 5) or 48 h (0 or 7) of stress