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Table 5 Differentially expressed proteins (DEPs) in the D. pulex clones G and M under heat-and-starvation stress

From: The transcriptomic and proteomic responses of Daphnia pulex to changes in temperature and food supply comprise environment-specific and clone-specific elements

Spot ID Gene ID Function R P SP Mre Mrp pIe pIp SC (%) NP
  24 h  
501 299795 3-Phosphoglycerate kinase 1.9 0.00 83.4 44.2 5.6 5.5 39.4 28
520 300012 Actin and related proteins 1.8 0.05 62.6 41.8 5.5 5.3 30.0 8
3309 306442 Actin and related proteins 1.4 0.02 62.6 41.9 5.4 5.2 30.8 8
543 220693 Arginine kinase 2.0 0.03 58.0 39.9 5.6 5.5 58.4 32
563 220693 Arginine kinase 3.1 0.03 57.6 39.9 5.6 5.5 58.4 32
796 220693 Arginine kinase 0.5 0.03 24.4 39.9 5.1 5.5 34.9 10
742 303899 Carboxypeptidase A2 0.6 0.02 17 30.0 46.1 4.8 5.2 29.6 14
383 301074 Chaperonin ATPase, Cpn60/Hsp60p 1.8 0.03 89.1 61.4 5.6 5.8 31.2 22
801 303282 Glutathione transferase 1.6 0.00 24.2 24.8 5.3 5.4 36.8 12
784 305501 Glutathione transferase 1.6 0.03 25.3 25.2 5.8 5.6 36.2 10
600 303036 Glycoside hydrolase, family 16 0.3 0.01 19 47.5 40.4 4.9 4.8 30.1 12
779 309746 H+-transporting two-sector ATPase 2.0 0.00 26.5 56.8 5.9 5.5 64.2 20
23,222 309746 H+-transporting two-sector ATPase 0.4 0.03 66.8 56.8 5.1 5.5 65.5 23
722 248155 Peptidase S1, chymotrypsin 2.0 0.02 15 39.9 30.2 5.5 4.7 22.3 3
845 9558 Ubiquitin and ubiquitin-like proteins 0.6 0.01 18.3 5.7 4.9 5.2 35.3 2
448 308693 Vitellogenin 0.5 0.01 20 74.3 191.9 5.5 6.5 9.8 31
Spot ID Gene ID Function R P R P SP Mre Mrp pIe pIp SC (%) NP
  24 h 48 h  
520 300012 Actin and related proteins 0.4 0.00 0.4 0.00 62.6 41.8 5.5 5.3 29.97 8
752 300012 Actin and related proteins 1.0 0.73 1.7 0.01 28.2 41.8 5.6 5.3 36.34 17
724 305550 Actin and related proteins 2.3 0.01 2.5 0.00 32.0 41.8 5.7 5.3 37.93 12
3309 306442 Actin and related proteins 1.4 0.19 1.5 0.03 62.6 41.9 5.4 5.2 30.77 8
405 301837 α-Tubulin 1.6 0.02 1.6 0.04 81.9 49.9 5.3 5.0 33.04 16
543 220693 Arginine kinase 1.7 0.06 1.9 0.03 58.0 39.9 5.6 5.5 58.38 32
563 220693 Arginine kinase 1.4 0.11 2.0 0.00 57.6 39.9 5.6 5.5 58.38 32
411 314456 β-Glucosidase 0.9 0.69 0.6 0.03 19 81.9 57.1 5.0 4.9 15.54 9
463 210624 Calreticulin 0.9 0.49 0.5 0.00 16 72.4 46.6 4.7 4.5 48.3 18
742 303899 Carboxypeptidase A2 0.9 0.51 0.5 0.00 17 30.0 46.1 4.8 5.2 29.59 14
383 301074 Chaperonin ATPase, Cpn60/Hsp60p 5.5 0.00 7.1 0.00 89.1 61.4 5.6 5.8 31.2 22
908 300446 Cytosolic fatty-acid binding protein 0.5 0.05 0.2 0.00 26.1 14.8 6.0 5.3 31.06 4
809 301844 Enolase 1.6 0.02 1.5 0.26 26.1 46.8 6.0 5.7 28.11 8
1027 301844 Enolase 1.1 0.53 1.4 0.05 5.0 46.8 6.6 5.7 19.82 6
805 231271 FKBP-type peptidyl-prolyl cis-trans isomerase 0.6 0.03 0.8 0.35 17 23.3 23.4 4.5 4.5 66.67 14
609 304126 FOG, leucine-rich repeat 3.2 0.02 3.5 0.00 22 47.2 42.1 4.6 4.7 18.64 7
801 303282 Glutathione transferase 1.7 0.04 1.3 0.13 24.2 24.8 5.3 5.4 36.77 12
784 305501 Glutathione transferase 3.0 0.00 3.4 0.00 25.3 25.2 5.8 5.6 36.24 10
794 317266 Glutathione transferase 1.6 0.02 1.6 0.04 25.0 25 5.4 5.2 67.26 18
600 303036 Glycoside hydrolase, family 16 0.8 0.35 0.5 0.01 19 47.5 40.4 4.9 4.8 30.08 12
415 306451 H+-transporting two-sector ATPase 3.2 0.01 9.1 0.00 66.9 55.5 5.7 5.5 57.6 56
466 309746 H+-transporting two-sector ATPase 1.2 0.39 1.7 0.02 71.4 56.8 5.2 5.5 79.4 56
779 309746 H+-transporting two-sector ATPase 2.9 0.02 3.1 0.01 26.5 56.8 5.9 5.5 64.23 20
23,222 309746 H+-transporting two-sector ATPase 1.0 0.80 0.4 0.00 66.8 56.8 5.1 5.5 65.54 23
332 200882 M13 family peptidase 0.6 0.07 0.3 0.00 99.0 75.4 4.7 4.6 47.86 34
635 251885 Serine endopeptidase 0.3 0.02 0.3 0.01 19 41.5 39.6 4.4 5.0 12.71 6
641 251885 Serine endopeptidase 0.2 0.01 0.3 0.00 19 41.2 39.6 4.5 5.0 12.71 6
444 9558 Ubiquitin and ubiquitin-like proteins 2.4 0.00 4.8 0.00 76.2 5.7 4.6 5.2 35.29 2
845 9558 Ubiquitin and ubiquitin-like proteins 0.7 0.09 0.5 0.00 18.3 5.7 4.9 5.2 35.29 2
231 308693 Vitellogenin 3.5 0.08 5.2 0.01 20 117.0 191.9 5.7 6.5 16.34 47
238 308693 Vitellogenin 3.7 0.13 2.9 0.04 20 117.0 191.9 5.8 6.5 18.39 46
270 219769 Vitellogenin fused with SOD 2.5 0.02 4.6 0.02 17 110.9 223 5.6 6.5 12.3 22
301 219769 Vitellogenin fused with SOD 3.9 0.01 3.9 0.00 17 107.0 223 5.6 6.5 13.25 34
305 219769 Vitellogenin fused with SOD 3.8 0.00 2.5 0.01 17 104.8 223 5.5 6.5 12.45 23
322 219769 Vitellogenin fused with SOD 3.0 0.01 3.5 0.00 17 102.6 223 5.6 6.5 12.25 21
325 219769 Vitellogenin fused with SOD 3.8 0.00 4.0 0.00 17 101.9 223 5.6 6.5 12.55 29
576 219769 Vitellogenin fused with SOD 2.4 0.01 3.6 0.00 17 51.9 223 5.7 6.5 7.5 18
580 219769 Vitellogenin fused with SOD 3.3 0.00 7.0 0.00 17 51.8 223 5.6 6.5 9.85 22
616 219769 Vitellogenin fused with SOD 1.3 0.32 2.1 0.01 17 45.1 223 5.6 6.5 8.9 24
689 219769 Vitellogenin fused with SOD 0.7 0.55 0.2 0.02 17 38.1 223 5.7 6.5 12.85 25
1545 219769 Vitellogenin fused with SOD 3.1 0.02 6.7 0.02 17 51.8 223 5.6 6.5 9.85 22
  1. D. pulex clone G on top, D. pulex clone M at the bottom. From left to right: Spot ID, gene ID, protein function, expression ratios (R) contrasting stress (24 h or 48 h at 30 °C, starvation) and control (20 °C, ad libitum feeding) conditions and associated P values (t-tests), predicted length of N-terminal signal peptides (SP), experimental and predicted molecular masses (Mre, Mrp) and isoelectric points (pIe, pIp) of the mature protein, sequence coverage (SC), and number of matching peptides (NP). Bold R and P values indicate statistical significance. (Clone G did not survive 48 h of heat-and-starvation stress.) See Table 3 for details. Number of gels (biological replicates, 25-30 animals each) for clone G or M: control (5 or 4) and 24 h (5 or 5) or 48 h (0 or 7) of stress