Fig. 2
From: SWI/SNF regulates half of its targets without the need of ATP-driven nucleosome remodeling by Brahma
The confident BRM target genes in S2 cells. Control cells and cells stably transfected with expression vectors for recBRM-V5 and recBRM-K804R-V5 were treated with dsRNA to knock down BRM. Control cells were treated in parallel with dsRNA complementary to GFP. The expression of the recombinant proteins was induced with CuSO4, as indicated. a Western blot analysis of BRM levels using an antibody against the endogenous BRM. Tubulin was used as a loading control. b Box plot showing the average expression levels of the BRM target genes compared with the average expression level of all the genes in S2 cells. c-d Metagene analyses of RNAPII and nucleosome occupancy in the BRM target genes (red, n = 541) and flanking sequences (X-axis) compared with the average distributions in all the S2 genes (blue, n = 13,294 genes)