Fig. 5

Cellular localization of Borealin, Gtsf1 and ZarI in tilapia testis and ovary by ISH. Borealin was weakly expressed in the oogonia, highly expressed in the phase I and II oocytes of the ovary, but was not expressed in the phase III and IV oocytes (a), while no Borealin expression was found in the testis (d). Gtsf1 was weakly expressed in the oogonia and phase I oocytes, highly expressed in the phase II oocytes, and moderately expressed in the phase II oocytes of the ovary (b), while no Gtsf1 expression were detected in the testis (e). ZarI was highly expressed in the phase I and II oocytes, and weakly expressed in the phase III oocytes in the ovary (c), and moderately expressed in the spermatocytes in the testis (f). OG, oogonia; OC, oocytes; I-IV, phase I to phase IV oocytes; SG, spermatogonia; SC, spermatocytes; Arrowheads indicate the positive signal