Fig. 1
Position and instability of (GT)n motifs in CGF and JGF goldfish strains. a Comparison of paternal and maternal promoters of ntl gene in goldfish intraspecific heterozyote. Black lines represent the conserved regions. Orange and blue boxes represent paternal and maternal (GT)n motifs, respectively. Orange and blue lines represent the paternal and maternal specific inserted sequences, respectively. Orange and blue dotted lines represent the paternal and maternal specific deleted sequences, respectively. Orange and blue arrow heads indicate the paternal and maternal specific SNP sites, respectively. b, c, Gel electrophoretogram of PCR products amplified from CGF and JGF genome DNA, respectively. M, markers. 1-10, the number of the samples. d Gel electrophoretogram of PCR products of (GT)n motif 1 in heterozygous embryos at different developmental stages. M, marker. PC, paternal control. MC, maternal control. G, gastrula stage embryos. B, bud stage embryos. D1-D5, 1-day to 5-day old embryos. P + M, mixed sample of equal paternal and maternal genomic DNA. Red and green arrow heads indicate the PCR products identical to paternal CGF and maternal JGF (GT)n motif 1, respectively. Black and white arrow heads indicate the PCR products of (GT)n motif 1 detected only in the embryo samples and detected in both the embryo and mix control samples, respectively