Fig. 4

Detection of transgene expression in SNU-F1–1. a Schematic of Dre-rox recombination in constructs used in this study. b Image of SNU-F1–1 (left) and its mother, SNU-SB-1 (right). c Primary cells from the ear skin of SNU-F1–1 express the transgenic reporter protein, GFP (upper). RFP expression is detected following transfection of ear skin cells with Dre recombinase (lower). d PCR analysis of Dre-rox recombination using genomic DNA from the cells derived from SNU-F1–1. P/C, positive control (PB-CA-Rox-GFP-Rox-RFP vector); WT, genomic DNA from wild type cattle; Tg:SNU-PB-1, genomic DNA from the blood of SNU-PB-1; Tg:SNU-F1–1, genomic DNA from the blood of SNU-F1–1; Tg:SNU-F1–1 + Dre, genomic DNA from cells from SNU-F1–1 which have undergone transfection with Dre recombinase; N/C, negative control (nuclease-free water)