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Table 1 Samples and experiments

From: A multi-scale analysis of bull sperm methylome revealed both species peculiarities and conserved tissue-specific features

Experiment Species Breed/strain Sample type Sample number Figures
LUMA Cattle Holstein PBMCs 73 Fig. 1a
Cattle Belgian White Blue Sperm, frozen 14 Fig. 1a-b, e
Cattle Holstein Sperm, frozen 112 Fig. 1a-b, e
Cattle Montbéliarde Sperm, frozen 41 Fig. 1a-b, e
Cattle Normande Sperm, frozen 18 Fig. 1a-b, e
Cattle Montbéliarde Sperm, frozen 6 Fig. 1c-d
Cattle Montbéliarde Total blood 6 Fig. 1c
Sheep Ile-de-France Sperm, frozen 5 Fig. 1c-d, e
Sheep Ile-de-France Total blood 5 Fig. 1c
Cattle Montbéliarde Sperm, fresh 6 Fig. 1d
Sheep Ile-de-France Sperm, fresh 4 Fig. 1d
Horse Welsh Sperm, fresh 2 Fig. 1e
Pig mixed Sperm, fresh 3 Fig. 1e
Mouse C57Bl/6JOlaHsd Sperm, fresh 2 Fig. 1e
Human   Sperm, frozen 7 Fig. 1e
Goat Alpine Sperm, frozen 2 Fig. 1e
Genotyping Cattle Montbéliarde Sperm, fresh 2 Additional file 1: Figure S2
Cattle Montbéliarde Total blood 2 Additional file 1: Figure S2
MeDIP Cattle Holstein Sperm, frozen 4 Figs. 2, 3, 4, 6
Cattle Holstein Liver 4 Figs. 2, 3, 4, 6
Cattle Holstein Fibroblasts 3 Figs. 2, 3, 4, 6
RRBS Cattle Holstein Sperm, frozen 2 Figs. 2, 3, 5, 6
Cattle Holstein Monocytes 2 Figs. 2, 3, 5, 6
Cattle Holstein Fibroblasts 2 Figs. 2, 3, 5, 6
  1. All the samples were independent, except for (i) the sperm and blood samples in Fig. 1c-d that were collected on the same bulls and rams, and (ii) two fibroblast samples and two livers that were collected on the same animals. Two independent amplifications of the same fibroblast cultures were used for MeDIP and RRBS. The sperm and blood DNA samples used for genotyping were the same as used for LUMA, Fig. 1c-d. PBMCs: peripheral blood mononuclear cells