Fig. 1

Characterization of WGBS missingness. (a) WGBS coverage (sequencing depth) distributions across tissue types and samples, visualized as the proportion of the ~ 25.5 million autosomal CpGs lying in each coverage interval. Each column is one sample from that tissue type. (b) WGBS missingness is distributed across chromatin states in all tissues. The normalized fraction of total missingness (y-axis) was calculated as the number of CpGs in each chromatin state that had missing methylation (beta) values (sequencing depth < 10×) normalized by the total number of CpGs in that chromatin state for each tissue. Abbreviations: AN, adipose NGT; AT, adipose T2D; MN, muscle NGT; MT, muscle T2D; Isl., islets; NGT, normal glucose tolerance; T2D, type 2 diabetes