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Table 2 Features of the 20 differentially expressed proteins identified by MALDI-TOF-MS

From: Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content

Stage Protein spot No. Fold change-ratio (Fat/Lean) Accession No. (NCBInr/UniProt) Protein name Molecular weight (kDa) pI Protein score Protein score C.I.% Subcellular localization
E7 3 2.77 gi|71895483 lysyl-tRNA synthetase 68,330.4 5.89 271 100 Cytoplasm; Mitochondrion
4 2.35 Unknown protein       
9 1.54 Q9YHT1 Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial 74,024.6 6.65 63 94.987 Mitochondrion
10 1.91 Q5ZJ08 Tyrosyl-tRNA synthetase, cytoplasmic 59,703.1 6.24 76 99.743 Cytoplasm
12a 1.65 gi|326927880 PREDICTED: transketolase-like [Meleagris gallopavo] 87,487.4 8.17 125 100  
21a −1.67 gi|326936041 PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial-like, partial [Meleagris gallopavo] 16,841.6 6.9 99 99.971  
23 1.99 gi|57530789 Thioredoxin domain-containing protein 5 47,249.1 5.61 167 100  
27 −3.33 gi|45384226 Sulfotransferase 36,333.5 5.89 129 100  
33 −1.97 gi|45384222 Heat shock protein beta-1 21,715 5.77 135 100 Cytoplasm; Nucleus; Mitochondrion
35a −1.62 gi|109032822 PREDICTED: transcription factor BTF3-like [Macaca mulatta] 17,669.1 5.74 181 100  
40 4.02 O57535 Nucleoside diphosphate kinase 17,447.9 7.72 75 99.661 Cytoplasm; Cell membrane
47 −1.56 gi|50751047 PREDICTED: inosine triphosphate pyrophosphatase 22,533.4 5.8 142 100  
50 −1.53 gi|50755667 PREDICTED: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 20,769.2 5.98 181 100  
58 −1.6 O57535 Nucleoside diphosphate kinase 17,447.9 7.72 123 100 Cytoplasm; Cell membrane
E12 21a −2.13 gi|326936041 PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial-like, partial [Meleagris gallopavo] 16,841.6 6.9 99 99.971  
24 −2.52 gi|129293 Ovalbumin 43,195.6 5.19 197 100 Extracellular region
25 −2.71 gi|129293 Ovalbumin 43,195.6 5.19 370 100 Extracellular region
27 −3.09 gi|45384226 Sulfotransferase 36,333.5 5.89 129 100  
33 −1.67 gi|45384222 Heat shock protein beta-1 21,715 5.77 135 100 Cytoplasm; Nucleus; Mitochondrion
E14 6 −1.89 Unknown protein       
7 −2.12 P84407 Alpha-fetoprotein 72,857.6 6.26 66 97.655 Secreted
8 −2.39 P84407 Alpha-fetoprotein 72,857.6 6.26 71 99.187 Secreted
14 −1.85 gi|86129440 Coronin-1C 53,744.3 6,22 109 99.997 Intracellular
E17 31 −1.73 P08250 Apolipoprotein A-I 30,661.1 5.58 72 99.292 Secreted
57 3.67 gi|56119000 Intestinal fatty acid-binding protein 15,079.7 6.62 247 100 Intracellular part
E21 40 2.46 O57535 Nucleoside diphosphate kinase 17,447.9 7.72 75 99.661 Cytoplasm; Cell membrane
  1. arepresents proteins matched to other databases. Unknown protein indicates protein spots could not be identified