Table 3 Joint analyses on differentially expressed genes and proteins in qRT-PCR and comparative proteomics
Group | Stage | Protein name | Gene name | DEPs in comparative proteomics | Differential expression genes in qRT-PCR | Molecular function | Biological process |
|---|---|---|---|---|---|---|---|
Group I-subgroup 1 | E7 | Tyrosyl-tRNA synthetase, cytoplasmic | YARS | Y (Fat > Lean) | Y (Fat > Lean) | Aminoacyl-tRNA ligase activity | Amino acid synthesis |
E17 | Intestinal fatty acid-binding protein | FABP2 | Y (Fat > Lean) | Y (Fat > Lean) | Lipid binding, transporter activity | Lipid transport | |
Group I-subgroup 2 | E7 | lysyl-tRNA synthetase | KARS | Y (Fat > Lean) | Y (Lean > Fat) | Aminoacyl-tRNA ligase activity | Amino acid synthesis |
Group II-subgroup 1 | E7 | Thioredoxin domain-containing protein 5 | TXNDC5 | Y (Fat > Lean) | N (Fat > Lean) | Disulfide oxidoreductase activity | Protein folding |
E7 | Sulfotransferase | SULT | Y (Lean > Fat) | N (Lean > Fat) | Sulfotransferase activity, transferase activity | Sulfuryl transfer | |
E7 | PREDICTED: inosine triphosphate pyrophosphatase | ITPA | Y (Lean > Fat) | N (Lean > Fat) | Pyrophosphatase activity | Nucleotide metabolic process | |
E7 | PREDICTED: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 | NDUFB10 | Y (Lean > Fat) | N (Lean > Fat) | Binding, NADH dehydrogenase (quinone) activity | Energy metabolism | |
E7 | Nucleoside diphosphate kinase (protein spot 58) | NDK | Y (Lean > Fat) | N (Lean > Fat) | Nucleoside diphosphate kinase activity | Lipid transport | |
E12 | Heat shock protein beta-1 | HSPB1 | Y (Lean > Fat) | N (Lean > Fat) | Molecular chaperone-mediated protein folding | Lipid clearance | |
Group II-subgroup 2 | E7 | Nucleoside diphosphate kinase (protein spot 40) | NDK | Y (Fat > Lean) | N (Lean > Fat) | Nucleoside diphosphate kinase activity | Lipid transport |
E7 | Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial | SDHA | Y (Fat > Lean) | N (Lean > Fat) | Adenyl nucleotide binding, succinate dehydrogenase activity | Energy metabolism | |
E7 | Heat shock protein beta-1 | HSPB1 | Y (Lean > Fat) | N (Fat > Lean) | Molecular chaperone-mediated protein folding | Lipid clearance | |
E12 | Sulfotransferase | SULT | Y (Lean > Fat) | N (Fat > Lean) | Sulfotransferase activity, transferase activity | Sulfuryl transfer | |
E14 | Coronin-1C | CORO1C | Y (Lean > Fat) | N (Fat> Lean) | Cytoskeletal protein binding | Actin cytoskeleton organization | |
E17 | Apolipoprotein A-I | ApoA-I | Y (Lean > Fat) | N (Fat > Lean) | Cholesterol binding, cholesterol transporter activity | Lipid transport | |
E21 | Nucleoside diphosphate kinase (protein spot 40) | NDK | Y (Fat > Lean) | N (Lean > Fat) | Nucleoside diphosphate kinase activity | Lipid transport |