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Table 2 Percentage of input reads alignment from each bodily fluid

From: Diversity and signature of small RNA in different bodily fluids using next generation sequencing

Bodily Fluid

Blood

Leukocytes

Plasma

Serum

Saliva

Cell-Free Saliva

Urine

Cell-Free Urine

Ave

SDEV

Ave

SDEV

Ave

SDEV

Ave

SDEV

Ave

SDEV

Ave

SDEV

Ave

SDEV

Ave

SDEV

aInput (million reads)

15.7

2.3

12.5

1.0

12.4

6.7

9.5

1.5

10.4

1.1

10.9

1.4

14.1

2.6

15.1

4.2

Successfully clipped

94.4

0.7

63.4

4.6

78.7

20.7

80.7

5.3

82.1

12.2

78.3

2.1

83.2

9.4

82.1

8.8

Failed quality filter

0.2

0.0

0.1

0.0

0.1

0.0

0.1

0.0

0.2

0.0

0.2

0.0

0.1

0.0

0.1

0.0

Failed homopolymer filter

0.0

0.0

0.2

0.0

0.0

0.0

0.0

0.0

0.1

0.0

0.1

0.0

0.1

0.0

0.0

0.0

UniVec contaminants

0.4

0.0

0.7

0.3

0.8

0.9

0.5

0.2

0.6

0.4

0.6

0.2

0.5

0.3

0.6

0.4

rRNA

9.1

1.4

36.0

1.5

2.0

0.9

7.0

3.4

16.6

7.5

13.0

6.2

5.3

4.4

2.1

1.3

Reads used for alignment

84.7

2.2

26.4

2.8

75.8

21.0

73.1

7.6

64.6

19.5

64.5

7.2

77.2

14.0

79.3

9.8

Genome

82.2

1.9

22.7

2.3

69.0

26.7

58.0

9.5

15.7

5.3

14.2

3.3

56.1

31.7

53.7

33.6

miRNA sense

70.5

3.1

6.1

0.9

11.6

7.7

10.2

3.4

1.4

0.9

2.7

0.7

3.2

1.9

4.4

2.8

miRNAprecursor sense

0.2

0.0

0.1

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

tRNA sense

1.7

0.3

3.8

1.0

8.9

11.1

22.0

6.1

5.4

4.1

4.6

1.3

47.4

37.6

44.5

40.0

piRNA sense

1.5

0.5

1.2

0.2

4.9

3.3

1.1

0.4

0.1

0.0

0.1

0.0

0.1

0.1

0.1

0.1

Gencode sense

7.9

0.7

9.6

1.1

42.7

28.1

23.0

3.7

5.1

2.7

4.1

2.5

1.9

1.5

1.1

1.0

Not mapped to genome or transcriptome

2.5

0.2

3.8

0.5

6.7

5.8

15.1

5.3

48.9

19.7

50.3

10.1

21.2

17.8

25.6

23.9

  1. aNumber of million input reads obtained from sequencing of each sample and used in the alignment