Fig. 4

Validation of CASMIR annotation by miRDeep2 using read counts and AUC features. a IsomiR reads of each miRNA were summed and correlated with miRNA read quantified by miRDeep2. The two quantifications correlate significantly with spearman r = 0.973. Cancer AUCs generated by (b) canonical form reads, and c most abundant isomiR reads quantified by the isomiR pipeline correlate with AUCs generated by miRdeep2 reads with spearman r = 0.954 and 0.950, respectively, indicating the high consistency between the two workflows