Fig. 7

Amino acid alignment and nucleotide divergence rates (dN/dS) of CBF-A3 gene and homologous amino acid sequences. Shown are alignments of two haplotype AA sequences of CBF-A3 and nine homologue plant AA sequences. The numbers above the alignment indicate the sites of AAs. The black line above the alignment illustrates the PKK/RPAGRxKFxETRHP and DSAWR motif, the red line the AP2 domain, the black arrows the β-strands and the black spirals the α-helices. The red arrows label the AA changes based on significantly associated SNPs or indels. The plot below the alignment shows the nucleotide divergence rates (dN/dS). The black line describes the dN/dS ratio. The red dots between alignment and plot indicate sites with significant negative selection (P < 0.05)