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Table 1 Identification of differentially expressed proteins in Sf9 cells treated with the azadirachtin by LTQ-MS/MS

From: DnaJ homolog subfamily A member1 (DnaJ1) is a newly discovered anti-apoptotic protein regulated by azadirachtin in Sf9 cells

Spot no Protein name Fold change a) NCBI accession number DP b) AAC c)
(%)
MW
(Da)
PI Peptides identified
D1 DnaJ homolog subfamily A member 1 1.61 ± 0.13 gi|14053203 2 2.00 45134.93 6.38 SGNDLILR
D2 Ribosomal protein SA 2.85 ± 0.18 gi|54609281 3 7.84 33408.77 4.87 FAAHTGATPIAGR
D3 Actin 8.97 ± 0.21 gi|46371991 6 13.70 40603.37 5.46 GYSFTTTAER
D4 Actin 3.21 ± 0.11 gi|46371991 7 16.99 40603.37 5.46 GYSFTTTAER
D5 Actin 1.92 ± 0.08 gi|46371991 7 16.99 40603.37 5.46 GYSFTTTAER
D6 Beta-tubulin 10.43 ± 0.24 gi|74275413 3 10.14 33323.12 5.9 EVDEQMLNIQNK
D7 Proteasome zeta subunit 6.26 ± 0.23 gi|114050993 7 36.63 26874.64 4.98 LFQVEYAIEAIK
D8 P27BBP/eIF6-like 3.17 ± 0.14 gi|82880642 4 14.69 26304.74 4.63 VQFENNNEVGVFSK
D9 Translationally-controlled tumor protein homolog 2.38 ± 0.16 gi|74837218 10 41.86 19938.81 4.67 LVETYAFGDKK
D11 Proteasome subunit alpha type 6-A 1.73 ± 0.07 gi|114052160 3 13.41 27143 6.44 GTDAAVVAAQR
U1 Ribosomal protein L9 5.06 ± 0.19 gi|112983495 6 25.79 21377.03 9.94 MAPGVTVVNSPK
U2 Abnormal wing disc-like 2.80 ± 0.22 gi|153791847 5 27.92 17312.92 6.75 NIIHGSDSVESAK
  1. a) Fold change: D1-D11: the ratio of protein intensity of CK versus AZA; U1-U2: the ratio of protein intensity of AZA versus CK.
  2. b) Distinct peptides matched. c) Amino acids coverage.