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Table 1 Identification of differentially expressed proteins in Sf9 cells treated with the azadirachtin by LTQ-MS/MS

From: DnaJ homolog subfamily A member1 (DnaJ1) is a newly discovered anti-apoptotic protein regulated by azadirachtin in Sf9 cells

Spot no

Protein name

Fold change a)

NCBI accession number

DP b)

AAC c)

(%)

MW

(Da)

PI

Peptides identified

D1

DnaJ homolog subfamily A member 1

1.61 ± 0.13

gi|14053203

2

2.00

45134.93

6.38

SGNDLILR

D2

Ribosomal protein SA

2.85 ± 0.18

gi|54609281

3

7.84

33408.77

4.87

FAAHTGATPIAGR

D3

Actin

8.97 ± 0.21

gi|46371991

6

13.70

40603.37

5.46

GYSFTTTAER

D4

Actin

3.21 ± 0.11

gi|46371991

7

16.99

40603.37

5.46

GYSFTTTAER

D5

Actin

1.92 ± 0.08

gi|46371991

7

16.99

40603.37

5.46

GYSFTTTAER

D6

Beta-tubulin

10.43 ± 0.24

gi|74275413

3

10.14

33323.12

5.9

EVDEQMLNIQNK

D7

Proteasome zeta subunit

6.26 ± 0.23

gi|114050993

7

36.63

26874.64

4.98

LFQVEYAIEAIK

D8

P27BBP/eIF6-like

3.17 ± 0.14

gi|82880642

4

14.69

26304.74

4.63

VQFENNNEVGVFSK

D9

Translationally-controlled tumor protein homolog

2.38 ± 0.16

gi|74837218

10

41.86

19938.81

4.67

LVETYAFGDKK

D11

Proteasome subunit alpha type 6-A

1.73 ± 0.07

gi|114052160

3

13.41

27143

6.44

GTDAAVVAAQR

U1

Ribosomal protein L9

5.06 ± 0.19

gi|112983495

6

25.79

21377.03

9.94

MAPGVTVVNSPK

U2

Abnormal wing disc-like

2.80 ± 0.22

gi|153791847

5

27.92

17312.92

6.75

NIIHGSDSVESAK

  1. a) Fold change: D1-D11: the ratio of protein intensity of CK versus AZA; U1-U2: the ratio of protein intensity of AZA versus CK.
  2. b) Distinct peptides matched. c) Amino acids coverage.