Fig. 2

Comparison of CpG methylomes between two samples. a Overall reads coverage of reduced representation bisulfite sequencing (RRBS) at CpGs between the twins. The RRBS strategy shows a high covered rate of CpGs in two samples. 10.88% and 10.72% of all human CpGs are sequenced by more than 5 reads (5×) in D3 and D4, respectively. b Overall distribution of CpGs’ methylated levels in D3 and D4. This violin plot shows that most CpGs are 100% methylated or 0% methylated, and the global methylation level is unchanged between the pair (Wilcoxon test non-significant). c Average CpGs’ methylation profile in gene body, upstream (− 2 kb of TSS) and downstream (+ 2 kb of TTS). d Circos plot shows the distribution of the DMRs along the genome. The tracks from outsider to inner: CpG islands downloaded from UCSC (green); the –log10 p-value of detected DMRs (blue); the average methylation levels per 1 kb in D3 (red); the average methylation levels per 1 kb in D4 (green). e Scatter diagram of all CpGs’ methylation levels calculated by RRBS data between two samples, D3 and D4, showing a similar overall CpGs methylation levels with Pearson correlation coefficient equal to 0.9613