Fig. 6

DNA methylation and gene expression detection of ZIC3 and NR2F2 from clinical cases. (a and c) Statistical summaries about DNA methylation status of DMRs in ZIC3 and NR2F2 in 20 clinical samples, consisting of five normal providers and fifteen DORV patients. (b and d) Diagrams exhibiting average methylated levels of individual CpG sites in DMRs of ZIC3 and NR2F2 from the indicated groups, respectively. (e and f) Histograms showing relative gene expression levels of ZIC3 and NR2F2 in different groups of specimens. (g and h) Scatterplots showing the gene expression levels of ZIC3 (g) and NR2F2 (h) are negatively correlated with their promoter methylation status. Pearson’s correlation coefficient and p-values were listed above the plot