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Table 4 qPCR analysis of imprinted genes and genes with ART-induced gene body methylation aberrancies

From: Intragenic sequences in the trophectoderm harbour the greatest proportion of methylation errors in day 17 bovine conceptuses generated using assisted reproductive technologies

Gene symbol (Chromosome) Differential Methylation EDMA NCBI Ref Seq ID SOV TE SOV TP IVP TE IVP TP
TCEB3 (chr 2) Gene body SOV TE and IVP TE NM_001102333.1 0.047 0.34 0.19 0.51
OCRL (chr X) Gene body TE IVP and TE SOV NM_001102191.2 0.08 0.25 0.12 0.28
ATP1A1 (chr 3) Gene body TP SOV and TP IVP NM_001076798.1 0.70 0.67 0.92 0.89
SNRPN (chr 21) N/A NM_001079797.1 0.63 0.51 0.15 0.16
H19 (chr 29) N/A NR_003958.2 0.62 0.32 0.88 0.45
  1. [P-values are given, significant values (p ≤ 0.05 unpaired, two tailed t-test) are underlined in bold]
  2. The downwards arrow represents that the gene is downregulated compared to control (AI)