Table 2 Eighteen polymorphic SSR markers validated in the 44 pear accessions
Marker | Repeat motif | Primer sequence (5′ → 3′) | Ta (°C) | Expected length (bp) | Actual length (bp) |
|---|---|---|---|---|---|
Pb3L11N5758 | (aat)14 | F: GGTGCTGTAAGGTGGTGATG R: ATGAAACCAGCCAAACAACC | 58 | 224 | 183–225 |
Pb3L4N5130 | (aat)16 | F: GCCTCCAGACTCAAACTTTCC R: TGCGAGAGCGTAGGAGAAAT | 56 | 229 | 205–244 |
Pb2LUN25693 | (ac)23 | F: CTTCGAAAGGTTCCCAGAAG R: AAGTACGGACCAAGGTGCAG | 53 | 200 | 169–225 |
Pb2L10N09474 | (ac)23 | F: CATAAGCACCCAAACTCGTG R: GCCATGAGTCAAAGAGTCCAG | 58 | 244 | 208–250 |
Pb3L15N3832 | (aca)10 | F: AATAGCGCATAGATCCAACCA R: CCTCCCCATAAGCTTCATCA | 58 | 203 | 183–207 |
Pb3LUN6782 | (aca)13 | F: TGCTTTACAGCTGAGCTTCG R: AAGTTTCCCTCGGGGTTTTA | 58 | 213 | 184–211 |
Pb2L15N13836 | (ag)20 | F: CGTCTTGAACCACCATCTCC R: CCACTTTCTTCCACCACCAT | 56 | 199 | 173–211 |
Pb2L2N00815 | (ag)20 | F: CCAAACACATGACCACAAGC R: AAATGGAGCAGGTGGGAGTA | 56 | 192 | 164–198 |
Pb2L10N09198 | (ag)20 | F: GAGAAATGCAGTGGGGATTG R: AAAGTGACCGCTCAAAATGC | 56 | 205 | 179–207 |
Pb2LUN24134 | (ag)21 | F: AAGCTGGTTATGTGGCTGCT R: ATTGAGTTCGCTCGTTCGTT | 56 | 204 | 171–197 |
Pb2L17N16884 | (ag)21 | F: TTGTGCCCTTTTTCCTACCA R: GGGCTAAACGCTTTGATGTT | 58 | 197 | 107–147 |
Pb2L8N07068 | (ag)21 | F: AGGAGCTAGCATGCTTTGGA R: CGACACGAACACAAGAAACA | 56 | 192 | 103–275 |
Pb2L1N00036 | (ag)22 | F: CACTATGCCAGTGACAAAGATTG R: AGTAGATGCTAGGGCCACCA | 58 | 198 | 174–214 |
Pb2L7N06609 | (ag)22 | F: ACCCTAGTCGTCGTTCTTGC R: GCAGAGACGGAAAGAAATGG | 56 | 207 | 149–207 |
Pb2L4N03431 | (ag)22 | F: CCCAAAAGTAACCGAGCGTA R: TTCAGCCAGCCACTCTCTCT | 56 | 191 | 168–200 |
Pb3L2N0143 | (aga)12 | F: ACCAGAACCCACTCACCATC R: CCGACAATAAAGGCCTCAA C | 56 | 198 | 178–199 |
Pb3L5N5166 | (aga)12 | F: CGAGAGCGCCTAAGAGAAGA R: CGGAGACTCGCTGACTCACT | 58 | 211 | 182–209 |
Pb4L1N0014 | (cata)11 | F: TCCCTTCAAGGCTGTGAGTT R: CAGAGGTTTGGTTTTGGTGA | 58 | 211 | 172–208 |