Fig. 1

Schematic outline of mouse experimental study. a Mouse breeding strategy to produce experimental (TRAMP x WSB) F2 mouse population. WSB male and female mice were bred to generate an F1 population of WSB mice. Male WSB F1 mice were then crossed with SV40 transgene positive TRAMP B6 female mice to generate an F2 population of WSB mice that are SV40 Transgene positive (Tg+). b A total of 307 male (TRAMP x WSB) F2 mice were maintained for 210 days, or until humane endpoints were reached, and sacrificed to quantify phenotypic traits of aggressive PC, including primary tumor and metastasis burden. These phenotypic traits were used further in genomic and transcriptomic analyses to identify loci associated with aggressive disease traits in (TRAMP x WXB) F2 mice