Fig. 9

Comparative analysis of PPARα-induced genes between different experiments. Transcriptomics was performed on livers of chimeric mice containing hepatocyte humanized livers. Mice were treated with 300 mg/kg fenofibrate daily for 4 days (n = 3) or vehicle (control, n = 3). A) The top 40 most significantly induced genes by fenofibrate in hepatocyte humanized livers were ranked according to IBMT P-value. In parallel, the expression profiles of the same genes in two independent microarray datasets are shown. The first dataset is derived from human precision cut liver slices treated with the PPARα agonist Wy-14,643 (100 μM) for 24 h (n = 4, GSE17251) [21]. The second dataset is derived from human primary hepatocytes treated with the PPARα agonist GW7647 (1 μM) for 24 h (n = 4, GSE53399) [56]