Fig. 3

Compromise of both cell polarity and actin cytoskeletal regulators results in dauer germline hyperplasia. a Canonical actin cytoskeleton regulators which were not identified in the screen were depleted using RNAi and germ cell nuclei counts were performed. Depletion of wsp-1, arx-2, arx-3 resulted in significant increase in the number of germ cells. b and c To test if par-4 mediates cell polarity independently of AMPK to establish dauer GSC quiescence, genes belonging to the cell polarity and cytoskeleton category were depleted in either a par-4 or an aak(0) background and germ cell nuclei counts were performed. **P < 0.001, *P < 0.05 when compared to L4440 using two-tailed t- test, n = 50