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Table 3 Candidate genes that suppress AMPK-dependent hyperplasia during the dauer stage

From: Genome-wide surveys reveal polarity and cytoskeletal regulators mediate LKB1-associated germline stem cell quiescence

Chromo-some

Cosmid identifier

Gene

Brief description

I

T12F5.4

lin-59

SET domain-containing protein, closely related to Drosophila tri-thorax ASH1 protein implicated in chromatin remodeling.

I

H15N14.1a

adr-1

Adenosine deaminase that acts on RNA by deaminating adenosines and generating inosines in dsRNA; protects transgenic RNA from RNAi silencing

I

C54G4.8a

cyc-1

Subunit of complex III cytochrome c reductase required for normal ATP production.

I

F26E4.9a

cco-1

Subunit of cytochrome c oxidase-1, a component of the electron transport chain in mitochondria

I

F35C12.1a

 

Uncharacterized

I

I-5 Q4 G9b

 

A vab-10 isoform. A spectraplakin, component of the hemidesmosome in C. elegans; required for transducing mechanical signals from the muscle cells to epidermis

I

E03H4.8

 

Uncharacterized

I

H28O16.1a

 

Uncharacterized

I

Y18D10A.13

pad-1

Unfamiliar conserved protein required for embryonic development

II

T08E11.5a

fbxc-19

F-box C protein

II

B0281.6

 

Uncharacterized

II

F42G2.4a

fbxa-182

F-box C protein

II

K02F6.1

 

Uncharacterized

II

T24E12.9a

 

Uncharacterized

II

F29G1.3a

vps-35

Vacuolar protein sorting factor

II

T02G5.9a

kars-1

Lysyl(K) Amino-acyl tRNA Synthetase

II

F22B5.2

eif-3.G

Encodes a homologue of eukaryotic translation initiation factor 3, subunit 4. Affects embryonic viability, fertility and growth

II

C50E10.2a

 

Uncharacterized

II

C50E10.3a

sre-53

Serpentine receptor, Class E

II

Y53F4B.g

 

Uncharacterized

III

H19M22.2a

let-805

Myoactin; subunit of the hemidesmosome complex

III

T20B6.3

 

Uncharacterized

III

F26A1.13a

 

Uncharacterized

III

B0336.2

arf-1.2

ADP-ribosylation factor homologue, a GTPase that regulates intracellular trafficking and the actin cytoskeleton

III

C28H8.11a

tdo-2

Tryptophan 2,3-DiOxygenase

III

T12A2.2a

stt-3

Yeast oligosaccharyltransferase subunit homologue

III

C18F10.4a

srg-1

Serpentine Receptor, Class G

III

F23F12.6

rpt-3

A triple A ATPase subunit of the 26S proetosome’s 19S regulatory particle base subcomplex; functions as a reverse chaperone by unfolding substrates and translocating them into the core proteolytic particle (CP) of proteasome

III

R13A5.7a

 

Uncharacterized

III

K12H4.4a

 

Uncharacterized

III

F02A9.6

glp-1

Notch receptor homologue required for germline stem cell mitotic proliferation

III

F54G8.1a

irld-34

Insulin/EGF-receptor L Domain protein

III

R10E11.2a

vha-2

V-ATPase subunit involved in protein sorting and receptor-mediated endocytosis

IV

F47C12.6

 

Uncharacterized

IV

W03B1.6a

oac-51

O-ACyltransferase homologue

IV

F57H12.1

arf-3

ADP-ribosylation factor homologue, a GTPase that regulates intracellular trafficking and the actin cytoskeleton

IV

Y43E12A.1

cyb-2.1

A cyclin B isoform

IV

C28C12.11a

 

Uncharacterized

IV

K07F5.7

 

Uncharacterized

IV

C04G2.11a

irld-21

Insulin/EGF-receptor L Domain protein

IV

T17B5.1a

 

Uncharacterized

IV

F25H8.3

gon-1

A metalloprotease involved in ECM degradation; controls gonadal morphogenesis

V

F33E11.1a

nhr-15

A nuclear hormone receptor

V

F48G7.11a

nhr-190

A nuclear hormone receptor

V

C24B9.7a

srg-59

Serpentine Receptor, Class G

V

F35F10.3

 

Uncharacterized

V

F32D1.2

hpo-18

Hypersensitive to POre-forming toxin

V

T24A6.12a

srbc-69

Serpentine Receptor, Class BC

V

Y47D7A_1a43.d

 

Uncharacterized

V

C10G8.8a

 

Uncharacterized

V

F07G11.4

 

Uncharacterized

V

C54F6.10a

str-31

Seven-transmembrane G-protein coupled receptor

V

C12D5.7a

cyp-33A1

Cytochrome P450 family

V

C37C6.6a

mig-6

Similar to extracellular matrix proteins papilin and lacunin; required for DTC migration at all phases

X

C54D1.5a

lam-2

Subunit of laminin required for basement membrane integrity and gonad morphology

  1. The gene identity of each RNAi clone was determined by the database available with the commercially available Ahringer RNAi library. The brief descriptions for each gene locus are derived from WormBase or determined according to their functional homology
  2. aRNA candidates that potentially function germline non-autonomously
  3. bThese wells did not appear to be in the Ahringer library database. The RNAi clone from this well was sequenced and compared to other species to identify potential orthologues