Table 1 Primers of candidate PCR reference genes
Category | Gene Name | Sense Primer/Anti-sense Primer (5′-3′) |
|---|---|---|
Sugarcane | Acyl-CoA dehydrogenase family member 10, ACAD | CGTGGCATGGATCTGATGGT/AGCCTGCTCCAGTTCAATCC |
Sugarcane | Casein kinase I isoform delta-like, CK1δ | TCAAGGGCTACCTCCCTCTC/GCATTCTTCCCTTCCGCTCT |
Sugarcane | OTU domain-containing protein 5, OTU5 | TGGTGCAGAGCCCATTAACA/GCCTTCACCTGGTCCCTATC |
Sugarcane | 12-oxophytodienoate reductase 7, OPR7 | TGTTCATCGCTAACCCGGAC/TTAGGCTGGCCAAGGAATGG |
Sugarcane | Polyadenylate-binding protein 8, PABp8 | TTGGGACTCTGACTTCTGCC/CCAGTGACCTTTGCTGCTTG |
Sugarcane | Serine/arginine repetitive matrix protein 1, SARMp1 | TGGACTTGGTCAGTTGGAAACA/TGTTCCTGAAGCCTATGTTGCT |
Sugarcane | Glyceraldehyde-3-phosphate dehydrogenase, GAPDH | AGGACTCCAAGACCCTCCTC/CTTCTTGGCACCACCCTTCA |
Sugarcane | Eukaryotic elongation factor 1A, eEF1Aa | TTTCACACTTGGAGTGAAGCAGAT/GACTTCCTTCACAATCTCATCATAA |
Sugarcane | Cullin, CULa | TGCTGAATGTGTTGAGCAGC/TTGTCGCGCTCCAAGTAGTC |
Sugarcane | Clathrin adaptor complex, CACa | ACAACGTCAGGCAAAGCAAA/AGATCAACTCCACCTCTGCG |
Sporisorium scitamineum | Conserved hypothetical protein, S2 | ACCTCGAGCAGCAACAGTG/ACCACAATCCAGAACTCGACG |
Sporisorium scitamineum | Conserved hypothetical protein, S4 | GACGGTGCCCAAGAACAGAG/CTGTGAGCTTCCAATTCCGC |
Sporisorium scitamineum | VPS73-protein involved in vacuolar protein sorting, S6 | AAAACCTAATGGTGGGCTCGG/GACCCAACCCGAACGAGAAC |
Sporisorium scitamineum | Synaptobrevin, S8 | TGCACAAGACCATCGACTCA/CGAGTTTTGCTTCTTGGCTGT |
Sporisorium scitamineum | GTP binding protein Rac1, S9 | CACGTGATATCCATGCGAACAAG/GAGAATGGTGCAGTTGTTCTTCT |
Sporisorium scitamineum | Inosine 5`-monophosphate dehydrogenase, S10 | CGTTGCAGGACATGGGTGTG/TTCTCGTAGCTGTGCAGACCA |
Sporisorium scitamineum | SEC65-signal recognition particle subunit, S11 | GAATGCTTGGAGGCATGGGG/GCGGGTTCATAGGGTCCTTC |
Sporisorium scitamineum | ADP-ribosylation factor, S12 | AACGACCGAGAGCGTGTTTC/AGCTTGTCCGTAATCTCGGC |