Identification and evaluation of PCR reference genes for host and pathogen in sugarcane-Sporisorium scitamineum interaction system

Table 2 The expression analysis of ten sugarcane candidate PCR reference genes (CRG) based on the RPKM value from RNA-seq data

CRG	YC05–179				ROC22				mean	SD	CV
CRG	CK-24 h	J-24 h	J-48 h	J-120 h	CK-24 h	J-24 h	J-48 h	J-120 h	mean	SD	CV
ACAD	37.82	37.89	34.78	37.11	37.01	34.56	35.16	36.61	36.37	1.35	3.70%
CK1δ	91.73	89.52	94.76	92.68	94.37	87.24	93.32	98.05	92.71	3.32	3.58%
OTU5	69.95	72.46	71.13	77.11	71.21	69.04	75.94	71.98	72.35	2.81	3.88%
OPR7	51.73	52.14	55.65	53.10	48.94	52.02	51.81	50.56	51.99	1.93	3.72%
PABp8	137.92	135.44	131.73	124.45	138.25	131.10	138.93	135.35	134.15	4.87	3.63%
SARMp1	31.50	32.97	31.20	32.83	32.69	31.46	31.33	30.79	31.85	0.85	2.65%
GAPDH	30.31	30.21	29.81	29.19	28.36	28.71	30.26	29.30	29.52	0.74	2.52%
eEF1A	2520.172	2233.869	2788.05	1980.246	2260.422	1920.52	2271.531	2100.286	2259.387	284.4435	12.59%
CUL	107.9021	105.826	101.8729	111.1481	117.8406	113.4682	118.8913	111.88	111.1037	5.800734	5.22%
CAC	–	–	–	–	–	–	–	–	–	–	–

Note: YC05–179 and ROC22, sugarcane genotype YC05–179 and ROC22; CRG candidate PCR reference gene, RPKM reads per kilo bases per million reads, CK/J-24 h, −48 h and − 120 h the data from the control/treated (CK/J) sample for CK/J-24 h, 48 h, 120 h incubation (Que et al. 2014), SD standard deviation, CV covariation, The CAC gene was not found in the RNA-seq data

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