Table 4 The length of qRT-PCR amplicon and the PCR efficiency of 18 candidates PCR reference genes (CRG)
Category | CRG | Amplicon length (bp) | E (%) | R2 |
|---|---|---|---|---|
Sugarcane | ACAD | 120 | 1.01 | 0.995 |
Sugarcane | CK1δ | 153 | 1.01 | 0.993 |
Sugarcane | OTU5 | 186 | 0.99 | 0.992 |
Sugarcane | OPR7 | 133 | 1.05 | 0.992 |
Sugarcane | PABP8 | 121 | 1.03 | 0.993 |
Sugarcane | SARMp1 | 102 | 1.01 | 0.999 |
Sugarcane | GAPDH | 170 | 1.04 | 0.990 |
Sugarcane | eEF1A a | 103 | 0.97 | 0.999 |
Sugarcane | CUL a | 105 | 1.06 | 0.999 |
Sugarcane | CAC a | 112 | 1.00 | 0.999 |
Sporisorium scitamineum | S2 | 118 | 1.04 | 0.999 |
Sporisorium scitamineum | S4 | 130 | 0.99 | 0.990 |
Sporisorium scitamineum | S6 | 138 | 0.96 | 0.995 |
Sporisorium scitamineum | S8 | 122 | 0.97 | 0.992 |
Sporisorium scitamineum | S9 | 143 | 1.04 | 0.993 |
Sporisorium scitamineum | S10 | 133 | 1.00 | 0.993 |
Sporisorium scitamineum | S11 | 102 | 1.00 | 0.995 |
Sporisorium scitamineum | S12 | 146 | 0.97 | 0.996 |