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Table 1 Comparison of RNA-seq and qPCR data

From: Cell type-specific analysis of transcriptome changes in the porcine endometrium on Day 12 of pregnancy

Ssc gene symbol

Ssc Entrez Gene ID

Hsa gene symbol

Hsa Entrez Gene ID

Hsa gene description

log2 FC P/C

P-value*

 
 

RNA-seq

qPCR

RNA-seq

qPCR

 
     

LE

GE

S

Complete

D12

D14

LE

GE

S

Complete

D12

D14

PubMed ID

ABCC1

733,619

ABCC1

4363

ATP binding cassette subfamily C member 1

3.5

1.4

1.1

2.4

1.6

–

< 0.001

0.224

0.321

< 0.001

< 0.01

–

27764917

LOC100738425

100,738,425

ABCC4

10,257

multidrug resistance-associated protein 4

nd

2.9

nd

2.2

2.1

–

–

0.019

–

0.001

< 0.001

–

24695625

ABCC9

100,127,449

ABCC9

10,060

ATP binding cassette subfamily C member 9

1.2

0.0

0.6

2.2

1.8

–

< 0.001

0.999

0.844

< 0.001

< 0.05

–

27764917

AKR1B1

396,816

AKR1B1

231

aldo-keto reductase family 1 member B

5.2

5.2

2.2

5.1

5.8

–

< 0.001

< 0.001

0.060

< 0.001

< 0.001

–

24695626

BAALC

100,170,128

BAALC

79,870

brain and acute leukemia, cytoplasmic

nd

nd

nd

−5.0

−5.8

–

–

–

–

< 0.001

< 0.001

–

24174570

LOC100157834

100,157,834

CDH17

1015

cadherin 17, LI cadherin (liver-intestine)

−6.5

−2.6

−5.8

−8.3

−6.3

–

< 0.001

0.001

< 0.001

< 0.001

< 0.001

–

24174570

CLDN1

100,625,166

CLDN1

9076

claudin 1

5.0

nd

4.2

3.4

1.8

–

< 0.001

–

< 0.001

< 0.001

0.002

–

25123632

PBD-2

404,699

DEFB1

1672

defensin, beta 1

−5.2

−1.7

−4.1

−6.4

−5.9

−3.4

< 0.001

0.015

< 0.001

< 0.001

< 0.001

0.010

24174570

FGF9

396,717

FGF9

2254

fibroblast growth factor 9 (glia-activating factor)

2.2

nd

nd

3.0

3.7

3.0

< 0.001

–

–

< 0.001

< 0.001

< 0.001

24174570

FGFR3

100,514,115

FGFR3

2261

fibroblast growth factor receptor 3

−1.2

−0.1

−1.1

−2.3

–

− 0.6

< 0.001

0.991

0.066

< 0.001

–

0.048

20393170

LOC100511475

100,511,475

FXYD4

53,828

FXYD domain containing ion transport regulator 4

−4.9

nd

−5.2

−7.4

−7.5

–

< 0.001

–

< 0.001

< 0.001

< 0.001

–

24174570

LOC100513220

100,513,220

GPR83

10,888

G protein-coupled receptor 83

−5.9

nd

−7.5

− 7.8

−4.0

–

< 0.001

–

< 0.001

< 0.001

< 0.001

–

24174570

HPGD

100,156,186

HPGD

3248

hydroxyprostaglandin dehydrogenase 15-(NAD)

−3.8

−1.4

−3.6

−3.0

−2.7

–

< 0.001

0.005

< 0.001

< 0.001

< 0.01

–

24695626

LOC100739374

100,739,374

IL24

11,009

interleukin 24

−2.8

−1.4

− 2.7

−4.6

−5.2

−5.3

< 0.001

0.254

< 0.001

< 0.001

0.024

0.033

24174570

IRF1

396,611

IRF1

3659

interferon regulatory factor 1

0.6

−0.1

0.4

0.4

0.5

2.9

0.115

0.890

0.397

0.087

0.188

0.003

24174570

IRG1

100,524,951

IRG1

730,249

immunoresponsive 1 homolog (mouse)

10.3

5.8

6.2

9.9

8.0

–

< 0.001

0.000

< 0.001

< 0.001

< 0.001

–

24174570

OSTN

100,049,691

OSTN

344,901

osteocrin

nd

nd

nd

10.3

12.4

–

–

–

–

< 0.001

< 0.001

–

24174570

S100A9

100,127,489

S100A9

6280

S100 calcium binding protein A9

12.4

11.5

11.3

10.4

10.6

9.2

< 0.001

< 0.001

< 0.001

< 0.001

< 0.001

< 0.001

24174570

SERPINB7

100,152,588

SERPINB7

8710

serpin peptidase inhibitor, clade B, member 7

10.4

7.9

6.2

10.3

8.9

5.4

< 0.001

< 0.001

< 0.001

< 0.001

< 0.001

0.018

24174570

SLCO2A1

100,144,510

SLCO2A1

6578

solute carrier organic anion transporter family member 2A1

1.0

0.4

2.3

0.5

3.0

–

0.011

0.960

< 0.001

0.053

< 0.001

–

24695625

SLCO4C1

100,737,557

SLCO4C1

353,189

solute carrier organic anion transporter family member 4C1

1.6

0.2

1.3

2.3

2.6

–

0.007

0.978

0.174

< 0.001

< 0.05

–

27764917

SLCO5A1

100,157,250

SLCO5A1

81,796

solute carrier organic anion transporter family member 5A1

8.4

4.9

5.9

6.4

9.2

–

< 0.001

< 0.001

< 0.001

< 0.001

< 0.05

–

27764917

SPP1

397,087

SPP1

6696

secreted phosphoprotein 1

4.1

1.6

3.2

2.4

2.5

3.2

< 0.001

0.005

< 0.001

< 0.001

0.008

0.029

24174570

LOC100738308

100,738,308

STAT1

6772

signal transducer and activator of transcription 1, 91 kDa

1.6

0.2

0.8

1.4

1.2

2.3

< 0.001

0.562

0.066

< 0.001

0.001

< 0.001

24174570

STC1

100,125,345

STC1

6781

stanniocalcin 1

−2.9

−1.0

−2.9

1.9

2.5

2.0

< 0.001

0.384

< 0.001

< 0.001

0.101

0.017

24174570

  1. Ssc: Sus scrofa; Hsa: Homo sapiens; P/C: pregnant vs. control; *for RNA-seq adjusted p-values; nd: not detectable
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BMC Genomics

ISSN: 1471-2164

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