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Fig. 3 | BMC Genomics

Fig. 3

From: Refining a steroidogenic model: an analysis of RNA-seq datasets from insect prothoracic glands

Fig. 3

Bombyx mori adenylate cyclases and 3′, 5′ cyclic nucleotide phosphodiesterases. a: Expression profile heat maps of Bm ACs-encoding genes during the final larval instar and the first day of the pupal stage. Abbreviations: V-0 to V-8: Days of the 5th Instar; P-0: First day of the pupal stage. b: Posterior probability tree of Bm and Dm AC proteins. Bombyx mori soluble guanylate cyclase (BmGCY) was used as an outgroup. Asterisks indicate the ACs that are expressed in Dm RGs based on RNA-seq data [31]. Numbers indicate the posterior probability score (%). c: Expression profile heat maps of Bm PDE-encoding genes during the final larval instar and the first day of the pupal stage. Abbreviations: V-0 to V-8: Days of the 5th Instar; P-0: First day of the pupal stage. d: Posterior probability tree of Bm and Dm PDEs. Dm phosphodiesterase 9 (CG32648) was used as an outgroup. Asterisks indicate the phoshodiesterases that are expressed in Dm RGs based on RNA-seq data [31]. Numbers indicate the posterior probability score (%). e: Effects of IBMX and Ionomycin on cAMP levels of PGs during the final larval instar and the first day of the pupal stage. Results are expressed as Mean ± SEM, n = 4–5. f: Effects of forskolin on cAMP levels of PGs during the final larval instar and the first day of the pupal stage. Results are expressed as Mean ± SEM, n = 4–5. g: Graphical representation of the cAMP signalling dynamics in Bm PGs. Transcript levels of BmAC1L (green circles) and BmPDE4L (red circles) were normalised against the peak value of each as 100% (V-4 for BmAC1L and V-7 for BmPDE4L). Dotted line indicates the ratio of normalised BmAC1L values/normalised BmPDE4L values. Results are expressed as Mean ± SEM, n = 4–6

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