Fig. 4

Expression profiling of putative lncRNAs under nitrogen (N) starvation and other stress conditions in rice. a Heatmap representation of low-coding potential novel transcripts differentially expressed in N-starved (-N_1d/3d/5d/7d) rice shoots and roots. b Heatmap representation of low-coding potential novel transcripts responding to various stress conditions. R, roots; S, shoots; Se, seedling. c Venn diagram of low-coding potential novel transcripts responding 7 day of N starvation (-N_7d) and phosphate starvation (-Pi_7d). Red arrow, > 2-fold up-regulation; green arrow, > 2-fold down-regulation. d, e, f Venn diagrams of putative lncRNAs responding to 7 days of N starvation (-N_7d), 7 days of phosphate starvation (-Pi_7d), and salt (d), cold (e), or drought (f) stress. g Read distribution of a N and phosphate (Pi) starvation-responsive putative lncRNA, Chr04G0017 in N-starved (-N) and Pi-starved (-Pi) rice. h Quantitative RT-PCR of Chr04G0017 in N-starved (-N) rice shoots and roots. Both shoots and roots are normalized relative to eEF-1α. N.D., not detected by qRT-PCR