Table 2 Candidate effectors from B. xylophilus: 54 genes are represented in the gland cell transcriptome (FPKM > 100) and have both a signal peptide and at least one occurrence of the STATAWAARS motif in the promoter region. The predicted protein domains were determined by BlastP (threshold of 1 e-04)
GeneID | Up-regulated in planta | Expression in planta | Expression in gland cells | Expression in gland cells confirmed | Predicted protein function |
|---|---|---|---|---|---|
BUX.s01281.223 | + | 103.9 | 20,907.19 | + | gi|171854685|dbj|BAG16532.1| expansin-like protein [B. xylophilus] |
BUX.s01063.193 | – | 1934.67 | 14,530.98 | gi|510850849|gb|EPB67435.1| Transthyretin-like family protein [Ancylostoma ceylanicum] | |
BUX.s01332.1 | + | 55.73 | 12,613.75 | Not known | |
BUX.s01639.10 | + | 55.73 | 12,613.75 | + | Not known |
BUX.s01144.234 | + | 171.06 | 8343.09 | + | gi|762079885|ref.|XP_011414138.1| PREDICTED: thaumatin-like protein 1b [Crassostrea gigas] |
BUX.s00532.10 | + | 128.23 | 7085.05 | gi|507051878|ref.|WP_016122867.1| LPXTG-domain-containing protein cell wall anchor domain [Bacillus cereus] | |
BUX.s00036.112 | + | 393.48 | 6830.64 | + | gi|50872001|dbj|BAD34545.1| beta-1,4-endoglucanase [B. xylophilus] |
BUX.s01259.45 | + | 84.44 | 6760.45 | + | gi|657202143|gb|AID50178.1| cysteine protease family cathepsin 1 [B. mucronatus] |
BUX.s00647.61 | + | 297.20 | 5542.85 | + | Not known |
BUX.s00036.113 | + | 322.72 | 4504.83 | + | gi|50871999|dbj|BAD34544.1| beta-1,4-endoglucanase [B. xylophilus] |
BUX.s01144.122 | + | 127.83 | 4148.47 | + | Not known |
BUX.s00713.953 | + | 176.45 | 3997.31 | Not known | |
BUX.s00139.22 | – | 2224.90 | 3881.41 | + | Not known |
BUX.s01147.176 | + | 85.58 | 3291.83 | gi|657202143|gb|AID50178.1| cysteine protease family cathepsin 1 [B. mucronatus] | |
BUX.s01662.95 | – | 251.02 | 2564.99 | gi|541044673|gb|ERG83573.1| vitellogenin-6 [Ascaris suum] | |
BUX.s01147.177 | + | 119.82 | 2190.46 | + | gi|657202143|gb|AID50178.1| cysteine protease family cathepsin 1 [B. mucronatus] |
BUX.s01066.8 | + | 987.43 | 2107.94 | gi|308493871|ref.|XP_003109125.1| CRE-LYS-8.1 protein [C. remanei] | |
BUX.s01063.106 | – | 45.41 | 1816.8 | + | Not known |
BUX.s01066.63 | + | 138.92 | 1437.37 | + | gi|68226394|dbj|BAE02683.1| beta-1,3-endoglucanase [B. xylophilus] |
BUX.s01144.305 | – | 25.97 | 1383.88 | Not known | |
BUX.c07686.1 | + | 5.18 | 1345.1 | Not known | |
BUX.s00713.1076 | – | 30.03 | 1270.5 | gi|657202143|gb|AID50178.1| cysteine protease family cathepsin 1 [B. mucronatus] | |
BUX.s01281.215 | + | 52.28 | 1076.62 | gi|171854689|dbj|BAG16534.1| expansin-like protein [B. mucronatus] | |
BUX.s01259.20 | + | 209.06 | 936.56 | + | gi|82175173|dbj|BAE48370.1| pectate lyase [B. xylophilus] |
BUX.s01259.83 | – | 21.80 | 785.02 | + | gi|657202143|gb|AID50178.1| cysteine protease family cathepsin 1 [B. mucronatus] |
BUX.s01259.22 | – | 6.95 | 708.17 | gi|82175173|dbj|BAE48370.1| pectate lyase [B. xylophilus] | |
BUX.s00116.606 | + | 33.60 | 677.13 | + | gi|402314083|gb|AFQ55440.1| venom allergen-like protein [Ditylenchus destructor] |
BUX.s01147.175 | + | 17.44 | 664.85 | gi|657202143|gb|AID50178.1| cysteine protease family cathepsin 1 [B. mucronatus] | |
BUX.s01259.23 | + | 34.02 | 649.9 | gi|82175173|dbj|BAE48370.1| pectate lyase [B. xylophilus] | |
BUX.s01254.165 | – | 122.36 | 606.76 | Not known | |
BUX.s01259.69 | + | 7.37 | 599.06 | Not known | |
BUX.s00647.68 | + | 50.73 | 586.73 | Not known | |
BUX.s00579.208 | – | 59.63 | 548.56 | Not known | |
BUX.c08842.2 | – | 3.06 | 497.53 | Not known | |
BUX.s01254.96 | – | 414.47 | 480.46 | Not known | |
BUX.s00116.607 | – | 26.29 | 460.6 | gi|657202143|gb|AID50178.1| cysteine protease family cathepsin 1 [B. mucronatus] | |
BUX.s00116.604 | + | 4.32 | 396.78 | gi|657202143|gb|AID50178.1| cysteine protease family cathepsin 1 [B. mucronatus] | |
BUX.s01518.90 | – | 0.95 | 395.68 | Not known | |
BUX.s01147.188 | – | 100.22 | 380.57 | + | gi|802707556|gb|KKA71696.1| vit-6, partial [Pristionchus pacificus] |
BUX.c08843.1 | 2.99 | 344.18 | Not known | ||
BUX.s00116.969 | + | 21.14 | 297.48 | + | Not known |
BUX.s00422.677 | – | 2685.78 | 289.34 | Not known | |
BUX.s01281.230 | + | 98.77 | 270.09 | + | gi|674842627|gb|AIL31417.1| expansin-like protein [B. xylophilus] |
BUX.s00116.597 | + | 1182.49 | 263.89 | gi|685827799|emb|CEF62721.1| Hypothetical protein SRAE_1000099100 [Strongyloides ratti] | |
BUX.s01066.145 | + | 68.54 | 201.78 | + | gi|68226394|dbj|BAE02683.1| beta-1,3-endoglucanase [B. xylophilus] |
BUX.s01066.65 | – | 0.09 | 185.69 | gi|68226394|dbj|BAE02683.1| beta-1,3-endoglucanase [B. xylophilus] | |
BUX.s00713.1002 | + | 28.14 | 168.39 | + | gi|541044223|gb|ERG83158.1| gut esterase 1 [Ascaris suum] |
BUX.s00358.21 | – | 536.25 | 152.96 | Not known | |
BUX.s00117.41 | + | 35.10 | 140.6 | Not known | |
BUX.c08842.1 | + | 13.08 | 123.5 | gi|657202143|gb|AID50178.1| cysteine protease family cathepsin 1 [B. mucronatus] | |
BUX.s00358.19 | – | 551.47 | 120.75 | + | Not known |
BUX.s00364.45 | + | 13.86 | 118.38 | Not known | |
BUX.s00036.107 | – | 0.52 | 117.17 | Not known | |
BUX.s00116.596 | + | 199.43 | 115.16 | Not known |