Generation of Mycoplasma hominis gene-targeted mutants by targeting-induced local lesions in genomes (TILLING)

BMC Genomics

Table 2 vaa- and oppA-mutants obtained by screening the 1200 EMS-generated M. hominis clone library

Mutant designation	Targeted gene	Nucleotide substitution^a	Amino-acid substitution^b	Comments
Mho66	vaa	G410726A	Glu54Lys
Mho119	vaa	C411332T	Leu328Phe
Mho787	vaa	C410526A	Pro59His
Mho864	vaa	C410197T	none	Mutation located upstream of the vaa gene (intergenic region)
Mho1055	vaa	C410154T	none	Mutation located upstream of the vaa gene (intergenic region)
Mho1133	vaa	G410726A	Gly126Ser
Mho258	oppA	G181068A	Leu813Leu	Silent mutation
Mho369	oppA	G180439A	Asp604Asn
Mho785	oppA	C181239T	Asp870Asp	Silent mutation
Mho940	oppA	G180634A	Glu669Lys

^aNucleotide substitutions are numbered according to the M. hominis PG21 genome (GenBank accession number FP236530)
^bAmino acid substitutions are numbered from the N-terminal end of the targeted protein

ISSN: 1471-2164