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Table 3 Mutations harboured by EMS-generated M. hominis mutants in comparison to the sequence of the M. hominis PG21 strain not exposed to EMS

From: Generation of Mycoplasma hominis gene-targeted mutants by targeting-induced local lesions in genomes (TILLING)

Mutant designation

Expected mutationa (gene, position in the gene)

Other mutations in the genomea (Locus, gene)

Number of additional mutations

Mho66

G410726A (vaa, 376)

none

0

Mho119

C411332T (vaa, 982)

- C230929T (intergenic)

- C316577T (MHO_2640, CHP)

- G463593A (MHO_3770, licA, Protein LicA homolog)

- G494526A (MHO_3990, nusG, Transcription antitermination protein)

4

Mho787

C410526A (vaa, 176)

- C5000T (MHO_0050, dnaN, DNA polymerase III beta chain)

- C44025T (MHO_0340, ktrA, Potassium uptake protein KtrA)

- C172081T (MHO_1460, rpsG, 30S ribosomal protein S7)

- C258548T (MHO_2150, rpoD, RNA polymerase sigma factor)

- C283090T (MHO_2320, ABC transporter, ATP-binding protein)

- G288972A (MHO_2370, tRNA/rRNA methyltransferase)

- C441828T (MHO_3650, asnA, Aspartate-ammonia ligase)

- C571686T (MHO_4600, pyrH, Uridylate kinase)

8

Mho1133

G410726A (vaa, 376)

- G179389A (MHO_1510, oppA)

- C206418T (MHO_1700, recU, Recombination protein U homolog)

- C381191T (MHO_3200, CHP, Hypothetical lipoprotein)

- G462316A (MHO_3760, gyrB, DNA gyrase subunit B)

- G490546A (intergenic)

- C644067T (MHO_5190, pip, Proline iminopeptidase)

6

Mho369

G180439A (oppA, 1813)

- G230779A (intergenic)

1

Mho940

G180634A (oppA, 2008)

- G136127A (MHO_1080, ATB-binding protein)

- G138607A (MHO_1090, HP)

- G203092A (MHO_1670, cmk, Cytidylate kinase)

- G211597A (MHO_1740, oppB, Oligopeptide transport system permease protein)

- A227310G (MHO_1880, aspS, Aspartyl-tRNA synthetase)

- G357674A (MHO_3060, leuS, Leucyl-tRNA synthetase)

- G371725A (MHO_3130, atpA, F1-likeX0)

- G649646C (MHO_5220, hsdS, Type I restriction enzyme specificity protein)

8

  1. amutations are numbered according to the M. hominis PG21 genome (GenBank accession number FP236530)
  2. CHP conserved hypothetical protein, HP hypothetical protein