A comparison of heat-stress transcriptome changes between wild-type Arabidopsis pollen and a heat-sensitive mutant harboring a knockout of cyclic nucleotide-gated cation channel 16 (cngc16)

Table 2 Examples of HS-dependent changes in non-coding RNAs

Gene ID	Description	WT Norm Avg.	WT Heat Avg.	log2- CHG	Sig. adj. p-val.	Mut Norm Avg.	Mut Heat Avg.	log2-CHG	Sig. adj. p-val.	Ratio of pollen to seedling^a
Micro RNAs, 6 total for WT, 4 shared by cngc16
AT2G25095	MIR156A	13.30	112.42	2.84	Y	18.26	80.45	1.99	Y	4
AT4G31877	MIR156C	2.64	17.79	2.17	Y	3.54	14.85	1.62	Y	1
AT2G39175	MIR160A	1.89	15.10	2.17	Y	7.75	12.67	0.62	N	3
AT2G25011	MIR836A	4.16	23.65	2.06	Y	7.03	18.07	1.18	N	330
AT2G24103	MIR831A	21.97	8.70	−1.21	Y	48.24	9.15	−2.23	Y	144
AT4G14811	MIR780A	131.10	47.38	−1.41	Y	219.82	66.31	−1.68	Y	52,756
Anti-Sense Long RNAs, 9 total for WT, 7 shared by cngc16
AT1G04533	overlaps AT1G06923	0.37	7.29	2.11	Y	0.00	4.52	1.68	N	NA
AT2G09430	overlaps AT2G43235	303.78	87.30	−1.72	Y	329.94	57.35	−2.41	Y	NA
Long Non-Coding, 40 total for WT, 31 shared by cngc16
AT5G04635	NA	12.13	317.30	4.36	Y	10.99	220.83	3.94	Y	NA
AT5G04815	NA	55.99	8.40	−2.50	Y	57.14	10.29	−2.29	Y	NA
Small Nucleoar and Nuclear RNAs, 10 total for WT, 7 shared by cngc16
AT4G04475	NA	0.00	6.28	2.24	Y	0.00	3.33	1.43	N	NA
AT3G57645	U2.2	15.21	1.26	−2.45	Y	38.85	5.27	−2.44	Y	20
Other RNAs, 14 total for WT, 10 shared by cngc16
AT5G28824	NA	0.00	21.53	3.50	Y	0.00	11.77	2.57	Y	17
AT1G64563	NA	1573.5	300.01	−2.33	Y	1130.83	187.96	−2.52	Y	15

^aRatio of expression between pollen and seedling is based on [22]
Norm Normal, Avg Average, log2-CHG log2-fold change, Sig. adj. p-val: significance based on adjusted p-value ≤0.01, Mut: cngc16, N No, Y Yes

ISSN: 1471-2164