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Fig. 5 | BMC Genomics

Fig. 5

From: Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers

Fig. 5

a Transcript abundance (FPKM) calculated by removing PCR duplicates using only mapping coordinates compared to using mapping coordinates and UMIs. b Using only mapping coordinates significantly biases against abundant and short genes. Outliers omitted. Wilcoxon rank sum test; n, number of genes in each group. c Relationship between cumulative coefficient of variation and transcript abundance

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