Fig. 6

Triacylglycerol (TAG) biosynthetic pathway genes are coordinately up-regulated in conidia. a Metabolic pathways leading toward TAG biosynthesis (left) and expressional heatmap of involved genes (right); log2 ratios of expression are plotted. Up-regulated genes (over 2-fold, relative to APP) are highlighted in blue (left) or ‘*’ (right). b Expressional validation of selected genes by qRT-PCR. Fold changes relative to conidia were plotted and error bars were calculated from three independent replicates. ‘*’ indicates significant down-regulation relative to CON at P < 0.05 using permutation test. ACC: acetyl-CoA carboxylase; AGAT: acylglycerol-phosphate acyltransferase; DGAT: diglyceride acyltransferase; FAS: fatty acid synthase; GPAT: glycerol-3-phosphate O-acyltransferase; GPDH: glycerol-3-phosphate dehydrogenase; MDH: malate dehydrogenase; ME: malic enzyme; PAP: phosphatidic acid phosphohydrolase; PC: pyruvate carboxylase; PDAT: phospholipid:diacylglycerol acyltransferase; PPP: pentose phosphate pathway; 9FADS: Delta-9 fatty acid desaturase