Fig. 8

Phosphate starvation responsive genes of C. fructicola are activated during the early infection phases. a qRT-PCR expressional profiles of one putative phytase and two putative phosphate transporters during apple (cv. Gala) leaf infection. Expression at different times after inoculation (black bars) was normalized to appressoria formed on onion epidermis (APP, white bar); error bars were calculated from three independent replicates. b Protein domain structures of the five glycerophosphodiester phosphodiesterase (GDPD) genes (GDPD1 to GDPD5) and their expressional profiles. In (a) and (b), ‘*’ indicates significant up-regulation relative to APP at P < 0.05 using permutation test