Fig. 6

CeHR-grown C. elegans exhibit higher number of physiological germ cell apoptosis, more pronounced alae structures, and smaller fat stores. a OP50 NGM, S-Medium and CeHR grown MD701 adults were stain with DAPI. One or two germ cells enclosed by CED-1::GFP are detected around the loop region of the gonads in MD701 adults grown in OP50 NGM and S-Medium (84.6 and 100% of the worms, respectively). CED-1::GFP is detected around a large proportion of germ cells in CeHR grown MD701 adults (90.9% of the worms). b Scanning electron microscope (SEM) micrographs of OP50 NGM and CeHR grown N2 and AB1 L3 animals. N2 and AB1 L3 animals display normal alae and annuli with grown on OP50 NGM (81.3 and 85.7% of the worms, respectively). N2 but not AB1 L3 exhibit more pronounced alae in CeHR (72.2 and 12.5% for N2 and AB1, respectively). c Nile red staining of L3 and adult N2 C. elegans grown on OP50 NGM and CeHR. The number of fat stores is indistinguishable between OP50 NGM and CeHR grown N2 animals, but the fat stores significantly larger in OP50 NGM (81.8% large; 18.2% small) than CeHR raised N2 adults (27.3% large; 72.2% small) around the abdominal and tail regions