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Fig. 2 | BMC Genomics

Fig. 2

From: Identification of microRNAs in response to aluminum stress in the roots of Tibetan wild barley and cultivated barley

Fig. 2

The difference in 1-kb insertion, HvAACT1 expression and organic acids secretion among three barley genotypes. a Detection of 1-kb insertion in the upstream of HvAACT1 coding region. b Expression level of HvAACT1. Two root segments (0-1 cm and 1-2 cm from tips) from four-day-old seedlings were sampled after exposure to 10 μM Al for 6 h. Actin was used as an internal control and expression relative to 1-2 cm root segments of Golden Promise (GP) is shown. Al-induced citrate (c), malate and oxalate (d) secretion. Root exudates were collected after four-day-old seedlings were exposed to 10 μM Al for 6 h. Data in b, c and d are means ± SD of three biological replicates and means labeled with different letters are significantly different at p < 0.05 by Tukey’ test

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