Fig. 1

DMSO-based differentiation protocol gives the best differentiation potential based on expression of differentiation markers and cell viability. PLB-985 cells were differentiated into a neutrophil-like state by culturing in different media (DMSO, ATRA + DMF, or dbcAMP) for 6 days. Undifferentiated cells were also analyzed. Cells were stained with an antibody against CD11b, chosen as an early differentiation marker (a), with FLPEP (a fluorescent ligand of FPR1), chosen as a late differentiation marker (b), or with NucRed Dead 647 Probe, to measure cell death (c). Samples were measured by cytometry and data was analyzed using MATLAB. d Cell growth was monitored at days zero, three, and six of the differentiation protocols, using the trypan blue dye exclusion test. The number of cells were normalized to the initial number of cells. These experiments were performed three times and a representative experiment is shown