A map of gene expression in neutrophil-like cell lines

BMC Genomics

Table 1 PLB-985 cell death and expression of FPR1 under several differentiation inducer agents

	In NucRed negative population
Differentiation protocol	% Cells expressing FPR1	Mean Fluorescence Intensity for FPR1	% Cell death (NucRed Dead +)
Undifferentiated cells	4	41	10
DMSO	48	100	8
ATRA + DMF	27	78	44
dbcAMP	75	382	58
DMSO + ATRA	6	47.4	66
DMSO + ATRA + CA	52	142^a	73
DMSO + Nutridoma CS	70	292.5	10

^aAutofluorescence in non-stained samples is very high, probably due to CA
PLB-985 cells were differentiated into a neutrophil-like state by culturing in the indicated different media for 6 days. Undifferentiated cells were also analyzed. Cells were stained with NucRed Deatd 647 Probe to measure cell death and with FLPEP, a fluorescent ligand of FPR1. Samples were measured by cytometry and data was analyzed using MATLAB. For the mean fluorescence intensity (MFI), values were normalized against the MFI of the sample of cells differentiated only with DMSO

ISSN: 1471-2164