Fig. 4

Genetic organization of the akb, phe, and prm genes in R. opacus R7. a On pPDG2 plasmid: akbA1a, large subunit of o-xylene dioxygenase; akbA2a, small subunit of o-xylene dioxygenase; akbA3, ferredoxin component; akbA4, reductase component; akbB, dihydrodiol dehydrogenase; akbS, sensor kinase; and akbT, response regulator. On pPDG5 plasmid: akbC, meta-cleavage dioxygenase, akbD, meta-cleavage hydrolase product, akbE, hydratase and akbF, aldolase. Dashed box reported the IS1415 insertion deriving from the transposon mutagenesis and dashed lines localized the insertion element within the akbS gene of R7. b Three phe gene clusters: pheA1a (P115), phenol hydroxylase, and pheA1b, phenol hydroxylase-reductase component located on the chromosome; pheA2a (P122), phenol hydroxylase, and pheA2b, phenol hydroxylase-reductase component located on the chromosome; pheA3a (P143), phenol hydroxylase, and pheA3b, phenol hydroxylase-reductase component located on the pPDG2 plasmid. c prm gene cluster located on the chromosome: prmA (P59), large hydroxylase subunit of a monooxygenase, prmC, small hydroxylase subunit of a monooxygenase, prmB, reductase component, and prmD, regulatory coupling protein. Genes with unknown or hypothetical functions were reported as HP. Identified genes (listed in Table 1) and their orientation are shown by arrow