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Fig. 1 | BMC Genomics

Fig. 1

From: Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping

Fig. 1

Multiple-sequence alignment of amplicons and cDNA clones. Multiple-sequence alignment of cDNA amplicons and cDNA clones (a and c) and Kos_KSP amplicons and clones (b and d). a In-Fusion cloning of cDNA amplicons generated from the serum of an infected pig. The cDNA amplicons used for the cloning is shown as the first sequence. b In-Fusion cloning of Kos_KSP derived amplicons. c TOPO-XL-2 cloning of cDNA generated from the serum of an infected pig. The sequences of two independent cDNA amplicons used for the cloning are shown d TOPO-XL-2 cloning of Kos_KSP derived amplicons. In a and c the top bars (light gray) depict differences from the consensus sequence at the nucleotide level, while the lower bars (dark gray) depict differences from the consensus sequence at the amino acid level

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BMC Genomics

ISSN: 1471-2164

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