Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping

Table 1 Efficiency of cloning full-length amplicons from Kos_KSP and cDNAs

Amplicon	Cloning method	# of clones	Positive/no. screened	#Correct insert size/#Screened with NotI	% correct of screened total	No. of clones sent for NGS
Kos_KSP	In-Fusion^d	232	12/24	12/12	50%	5
Kos_KSP	In-Fusion^d	111	8/24	8/8	33.3%	5
Kos_KSP	In-Fusion^b	472	24/24	6/6	100%	–
Kos_KSP	In-Fusion liq.^b	> 1000	10/10	10/10	100%	–
cDNA^a	In-Fusion^b	24	6/24	3/6	12.5%	3
Kos_KSP	TOPO XL-2^b	> 1000	n.d.	8/10	80%	7
Kos_KSP	TOPO XL-2^b	> 1000	n.d.	6/10	60%	4
cDNA^ac	TOPO XL-2^b	31	n.d.	4/10	40%	4
cDNA^ac	TOPO XL-2^b	43	n.d.	7/10	70%	7
cDNA^a	TOPO XL-2^b	33	n.d.	4/10	40%	4

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