Amplicon
|
Cloning method
|
# of clones
|
Positive/no. screened
|
#Correct insert size/#Screened with NotI
|
% correct of screened total
|
No. of clones sent for NGS
|
---|
Kos_KSP
|
In-Fusiond
|
232
|
12/24
|
12/12
|
50%
|
5
|
Kos_KSP
|
In-Fusiond
|
111
|
8/24
|
8/8
|
33.3%
|
5
|
Kos_KSP
|
In-Fusionb
|
472
|
24/24
|
6/6
|
100%
|
–
|
Kos_KSP
|
In-Fusion liq.b
|
> 1000
|
10/10
|
10/10
|
100%
|
–
|
cDNAa
|
In-Fusionb
|
24
|
6/24
|
3/6
|
12.5%
|
3
|
Kos_KSP
|
TOPO XL-2b
|
> 1000
|
n.d.
|
8/10
|
80%
|
7
|
Kos_KSP
|
TOPO XL-2b
|
> 1000
|
n.d.
|
6/10
|
60%
|
4
|
cDNAac
|
TOPO XL-2b
|
31
|
n.d.
|
4/10
|
40%
|
4
|
cDNAac
|
TOPO XL-2b
|
43
|
n.d.
|
7/10
|
70%
|
7
|
cDNAa
|
TOPO XL-2b
|
33
|
n.d.
|
4/10
|
40%
|
4
|
- n.d. Not done
- aRNA derived cDNA
- bGel purified amplicons
- cSame RT-PCR used
- damplicons purified by PCR clean-up kit