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Table 1 Efficiency of cloning full-length amplicons from Kos_KSP and cDNAs

From: Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping

Amplicon Cloning method # of clones Positive/no. screened #Correct insert size/#Screened with NotI % correct of screened total No. of clones sent for NGS
Kos_KSP In-Fusiond 232 12/24 12/12 50% 5
Kos_KSP In-Fusiond 111 8/24 8/8 33.3% 5
Kos_KSP In-Fusionb 472 24/24 6/6 100%
Kos_KSP In-Fusion liq.b > 1000 10/10 10/10 100%
cDNAa In-Fusionb 24 6/24 3/6 12.5% 3
Kos_KSP TOPO XL-2b > 1000 n.d. 8/10 80% 7
Kos_KSP TOPO XL-2b > 1000 n.d. 6/10 60% 4
cDNAac TOPO XL-2b 31 n.d. 4/10 40% 4
cDNAac TOPO XL-2b 43 n.d. 7/10 70% 7
cDNAa TOPO XL-2b 33 n.d. 4/10 40% 4
  1. n.d. Not done
  2. aRNA derived cDNA
  3. bGel purified amplicons
  4. cSame RT-PCR used
  5. damplicons purified by PCR clean-up kit