Table 1 Primers for RT-qPCR amplification of miRNAs
ID | Primer | Sequence (5′-3′) |
|---|---|---|
miR-7 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCAACAAA |
Forward | GTGGAAGACTAGTGATTTTG | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir16 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCTGAAC |
Forward | GCACCGAAGCTTAGGGTT | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir18 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAGAATAC |
Forward | GCCTATAATGGCTATTGGTA | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir19 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGCATCT |
Forward | ATCCTTGGACCACAGCAG | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir22 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACACCT |
Forward | TGAGGGTGACTGGCAGG | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir24 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTTCGGC |
Forward | CACCACTCTTGTCTCTGC | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir26 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCGAGT |
Forward | ATGATGGCAGCGGTGACT | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir31 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACTGAC |
Forward | CTAATTTGAGCCATCTGTCA | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir34 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCAAGATG |
Forward | CACAGCCGTGTAGTCATC | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir35 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTGGCCA |
Forward | GTATTGGGCGTGTGTTGG | |
Reverse | TGCGTGTCGTGGAGTC | |
.novel_mir37 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTGAGGA |
Forward | TCGCAGATCCAGAATGTTC | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir40 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACTGAAG |
Forward | TGGAATGCATGGCTACACT | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir45 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTGTCCG |
Forward | GGCGTGGCAGGGGTTTC | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir47 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCGAGT |
Forward | ATGATGGCAGCGGTGACT | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir48 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACAGGC |
Forward | TACCCTGATATTCCTTGCC | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir5 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTTTGGGA |
Forward | TCAATGCCCTTGGAAATCC | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir53 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCTTAGG |
Forward | GGGTTAGTCGGGTCCTA | |
Reverse | TGCGTGTCGTGGAGTC | |
novel_mir7 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCTCACA |
Forward | GATGACTACACGGCTGTG | |
Reverse | TGCGTGTCGTGGAGTC | |
18S rRNA | Forward | GGAATTCCCAGTAAGCGCAA |
Reverse | CCAGTCCGAAGGCCTCACTA |