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Fig. 1 | BMC Genomics

Fig. 1

From: Deciphering the oviductal extracellular vesicles content across the estrous cycle: implications for the gametes-oviduct interactions and the environment of the potential embryo

Fig. 1

Oviduct EVs characterization and quantification across the bovine estrous cycle. a Representative images of exosomes (blue arrows, 30–100 nm size) and microvesicles (red arrows > 100 nm size) across the different stages of the bovine estrous cycle (Stage 1, Stage 2, Stage 3 and Stage 4) by TEM. b Histograms showing the size distribution of oEVs across the estrous cycle. A batch of 12 samples containing 3 replicates/stage were analyzed using TEM and ImageJ software. Comparison between populations of exosomes and microvesicles for each stage were performed (*P < 0.05; **P < 0.01, T-test. c Western blotting characterization of bovine oEVs at all 4 stages analyzed for known exosomal protein markers. A pool of samples containing 3 biological replicates at the 4 different stages analyzed was used, showing oEVs from all 4 stages were positive for HSP70 and ANAX1.). d Bovine oEVs size average and concentration was measured by Nanosight analysis from a different batch of 12 samples (3 replicates /stage)

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BMC Genomics

ISSN: 1471-2164

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