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Table 1 DAVID functional annotation clusters for top 500 most abundant mRNA identified in oEVs

From: Deciphering the oviductal extracellular vesicles content across the estrous cycle: implications for the gametes-oviduct interactions and the environment of the potential embryo

Most descriptive categories of DAVID functional annotation clusters groups by similar GO terms #mRNA1 Score2
GO Group 1: Protein translation and transport 236 63
protein transport (145, 2.8)3, translation (129, 7.3), ribosome (87, 11.4), protein targeting to ER (81, 28.4), nuclear transport (29, 2.2), regulation of protein localization (40, 1.5)   
GO Group 2: Extracellular vesicles 254 59.4
extracellular exosome (253, 3), extracellular vesicle (253, 3), phagocytosis (13, 1.7)   
GO Group 3: Gene expression 314 46.5
RNA binding (203, 4.4), gene expression (244, 1.7), negative regulation of metabolic process (109, 1.6), negative regulation of transcription, DNA-templated (42, 1.4)   
GO Group 4: Cell adhesion 139 40.5
adherens junction (112, 5.3), focal adhesion (77, 6.5), cadherin binding (51, 5.9)   
GO Group 5: Mitochondrion/oxidation-reduction 131 7.4
mitochondrion (86, 1.7), generation of precursor metabolites and energy (37, 3.6), oxidation-reduction process (49, 1.8), ATP metabolic process (30, 4.5), oxidative phosphorylation (23, 4.2)   
GO Group 6: Apoptosis 87 6
programmed cell death (87, 1.7), apoptotic signaling pathway (40, 2.5), apoptotic mitochondrial changes (10, 3.1)   
GO Group 7: ATPase/GTPase 54 6
nucleoside-triphosphatase activity (51, 2.3), ATPase activity (23, 1.9), GTPase activity (21, 3.2), GTP binding (22, 2.0)   
GO Group 8: Response to stress 172 5
cellular response to stress (78, 1.6), response to oxidative stress (28, 2.6), antioxidant activity (11, 4.9), reactive oxygen species metabolic process (16, 2.6), regulation of response to DNA damage stimulus (10, 2.3)   
GO Group 9: RNA processing/transport 42 3.3
RNA splicing (29, 2.6), mRNA processing (25, 1.9), spliceosomal complex (14, 2.6), RNA localization (14, 2.4), nuclear export (10, 2.0)   
GO Group 10: Immune response, proteolysis 112 3.2
immune response (59, 1.4), regulation of proteolysis (40, 2.1), regulation of mRNA stability (23, 4.8), regulation of cytokine production (26, 1.6), platelet activation (13, 2.9), regulation of peptidase activity (23, 2.1)   
GO Group 11: Cell cycle 64 2.9
mitotic cell cycle (45, 1.7), regulation of cell cycle (40, 1.5)   
GO Group 12: Cytoskeleton 71 2.7
cytoskeleton (50, 1.9), microtubule (21, 1.7), cytoskeleton organization (51, 1.6), actin cytoskeleton organization (28, 1.8), actin filament organization (18, 2.0)   
GO Group 13: Signal transduction, phosphorylation 192 2.6
regulation of protein metabolic process (118, 1.7), signal transduction by protein phosphorylation (38, 1.6), intracellular signal transduction (88, 1.2), phosphorylation (75, 1.2), phosphorus metabolic process (105, 1.2)   
GO Group 14: Chromatin 23 2.6
chromatin DNA binding (11, 3.8), protein-DNA complex assembly (15, 2.3), chromatin assembly (9, 2.0)   
GO Group 15: Development 41 2.3
gland development (22, 1.9), reproductive system development (19, 1.6), developmental process involved in reproduction (25, 1.4)   
GO Group 16: Response to hormones, growth factors, nutrients 80 2.2
response to organic cyclic compound (42, 1.7), response to steroid hormone (20, 1.9), response to nutrient levels (19, 1.7), response to growth factor (28, 1.6), response to glucocorticoid (12, 3.1), response to cAMP (8, 3.0)   
GO Group 17: Cell organization 82 1.8
regulation of organelle organization (47, 1.5), regulation of cellular component organization (76, 1.2), cell leading edge (20, 1.9)   
GO Group 18: Others 68 2.6
energy reserve metabolic process (11, 4.3), glycogen metabolic process (10, 4.6), isomerase activity (13, 2.8), muscle contraction (17, 1.9), organic acid metabolic process (37, 1.5)   
  1. Table shows DAVID functional annotation clusters with a score ≥ 1.3. 1number of unique mRNA in each GO group;2enrichment score (geometric mean of member’s p-values of the corresponding annotation cluster in -log10 scale) of the annotation cluster; 3in brackets: number of genes and fold enrichment for the functional term